and rapid CAD progression. The hs-CRP levels predict future cardiovascular events independently of CAD severity. Therefore, hs-CRP levels may be considered as marker of atheromatous plaque vulnerability and CAD activity. EAS16-0776, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. INTERMEDIATE DENSITY LIPOPROTEIN IS ASSOCIATED WITH MONOCYTE SUBSET DISTRIBUTION IN PATIENTS WITH STABLE ATHEROSCLEROSIS K. Krychtiuk 1 , S. Kastl 1 , T. Pongratz 2 , G. Goliasch 1 , L. Gaspar 3 , G. Maurer 1 , K. Huber 4 , E. Dostal 2 , S. Pfaffenberger 1 , S. Oravec 3 , J. Wojta 1 , W. Speidl 1 . 1 Medical University of Vienna, Department of Internal Medicine II, Vienna, Austria; 2 Krankenanstalten Dr. Dostal, Krankenanstalten Dr. Dostal, Vienna, Austria; 3 Comenius University, 2nd Department of Internal Medicine, Bratislava, Slovakia; 4 Wilhelminenhospital, 3rd Department of Medicine, Vienna, Austria Objectives: Intermediate density lipoprotein (IDL) consists mainly of chylomicron remnants and very low densitiy lipoprotein (VLDL) remnants that are thought to be proinflammatory lipoprotein particles. Atheroscle- rosis is considered to be an inflammatory disease of the vessel wall in which monocytes and monocyte-derived macrophages are crucially involved. Circulating monocytes can be divided according to their surface expression pattern of CD14 and CD16 into at least three subsets with distinct inflammatory and atherogenic potential. The aim of this study was to investigate whether IDL is associated with proinflammatory monocyte subsets. Methods: We included 90 patients with stable coronary artery disease (CAD). Monocyte subsets were identified as classical monocytes (CD14++CD16-; CM), intermediate monocytes (CD14++CD16+; IM) and non-classical monocytes (CD14+CD16++; NCM) by flow cytometry. Lipo- protein subfractions were measured by an electrophoresis method on polyacrylamide gel. Results: IDL correlated significant with the proinflammatory IM (r¼0.24; p<0.05) whereas VLDL and low densitiy lipoprotein (LDL) were not asso- ciated with monocyte subtypes. IDL was not associated with CM (r¼-0.18; p¼0.09) and NCM (r¼0.16; p¼0.13) but correlated significant with the acute phase protein C-reactive protein (r¼0.40; p<0.01). The association of IDL with IM was independent of cardiovascular risk factors and statin treatment. Patients with IDL>median (38mg/dL) showed a significant higher proportion of IM as compared to patients with IDL<38mg/dL (5.6 IQR 4.3-8.3% vs. 4.1 IQR 2.6-6.2%). Conclusions: In conclusion, we provide a potential link between elevated levels of IDL and a proinflammatory distribution of monocyte subtypes in patients with stable atherosclerotic disease. This possible proatherogenic role of IDL warrants further studies. EAS16-0801, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. THE ROLE OF MYELOID DENDRITIC CELLS, CHEMOKINE CCL17 AND T- CELL RECEPTOR CCR4 IN ATHEROGENESIS A. Karpov 1 , A. Rvatcheva 2 , M. Shogenova 3 , R. Zhetisheva 3 , V. Masenko 2 , V. Naumov 3 . 1 Moscow, Russia; 2 Russian Cardiology Research and Production Complex, neurohormonal and immunological studies, Moscow, Russia; 3 Russian Cardiology Research and Production Complex, problems of atherosclerosis, Moscow, Russia Objectives: To explore the association between circulating myeloid den- dritic cells (mDC), chemokine CCL17 and CCR4 receptor on the surface of T- helper cells (CD4+CCR4+) with the severity of coronary atherosclerosis. Methods: The study involved 92 male patients aged 24 to 80 years, who underwent coronary angiography. Patients were divided into 2 groups based on the presence/absence of revealed hemodynamically significant coronary artery stenoses. The severity of atherosclerotic coronary lesions was evaluated using the Gensini score. All patients underwent determi- nation of circulating mDC ((CD14+CD16)-FITC/CD85k(ILT3)-PE/CD33-PC5) and CD4+CCR4+ in the peripheral blood by flow cytometry, chemokine CCL17 by quantitative Sandwich ELISA. Results: The patients with stenotic coronary atherosclerosis, compared with the control group, had significantly lower blood level of mDC (14.22 cells/ml and 18.71 cells/ml respectively, p¼ 0.038) and higher blood level of CD4+CCR4+ (376.96 cells/ml and 264.45 cells/ml, respectively, p¼ 0.024). We also found an inverse correlation between the blood levels of CCL17 and CD4+CCR4+ (p¼ 0.033). The average value of the Gensini score in the study group was 39.91 (0-182.0) in the control group- 0.74 (0-5.0). Conclusions: The study demonstrated, that the patients with severe atherosclerotic coronary disease have a lower content of mDC and a higher content of CD4+CCR4+ in peripheral blood. The blood level of CCL17 has inverse correlation with expression of CCR4 by T-helper cells. The identi- fied changes can help better understanding of the immunoinflammatory component in atherogenesis. EAS16-0872, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. THE INFLAMMATORY PROCESS AS A RISK FACTOR OF DEVELOPING ATHEROSCLEROSIS IN PATIENTS WITH RHEUMATOID ARTHRITIS I. Starodubtseva. Voronezh, Russia Objectives: It is well-known that the main causes of mortality in RA pa- tients are cardiovascular events (Michaud K., Wolfe M., 2007). Chronic inflammation in RA patients is one of the main causes of atherosclerosis formation. Proinflammatory cytokines, especially IL-1 plays a significant role in forming atherosclerosis (Mazur V., 2005). Methods: 620 patients with RA (diagnosis according to ACR /EULAR) from the rheumatology in-patient clinic with the mean age of 43.4±10; 95.4 % ACCP e positive patients, activity on DAS 28 II, III; 85.4 % female with the disease duration for about 3-15 years were enrolled in the trial. We assessed the level of IL-1 with the use of ELISA. Results: The analysis of the results showed the statistically significant correlation of TG, LDG and markers of inflammation IL-1, DAS 28: p¼0.627 (p<0.01), p¼0.527 (p<0.01), p¼0.712 (p<0.01), p¼0.776 (p<0.01) accord- ingly. The correlation coefficient between hs-CPR and the indicators of the lipid profile revealed similar interconnections. The constructed model surfaces indicated the interdependence of IL-1, the activity of DAS 28 and the level of LDH in RA patients. Conclusions: According to the index of disease activity on DAS and markers of inflammation (IL-1 and hs-CRP) the severity of inflammatory process in RA patients is a risk factor for developing atherosclerosis. The analysis of inflammation indicators in RA patients allows to assess the risk of developing and progressing atherosclerosis. The data enables to select the best possible personified therapy for such patients at the early stage of the disease. EAS16-0920, VASCULAR BIOLOGY: MACROPHAGES, INFLAMMATION, IMMUNITY. MMP-12 AND TIMPS BEHAVIOUR IN STROKE RELATED TO CAROTID ARTERY STENOSIS N. Cifani 1 , F. Del Porto 1 , L. Ferri 1 , M. Proietta 1 , D. Toni 2 , R. Dito 1 , M. De Michele 2 , A. Falcou 2 , M. Taurino 1 . 1 Sapienza Universit a di Roma, Dipartimento di Medicina Clinica e Sperimentale, Roma, Italy; 2 Sapienza Universit a di Roma, Dipartimento di NEUROLOGIA E PSICHIATRIA, Roma, Italy Objectives: the aim of this study was to evaluate metalloproteases (MMP)- 12 levels and its tissue inhibitors (TIMP)-1, -2,-3 and-4 in 30 patients: 15 affected by stroke related to carotid artery stenosis (CAS) and 15 with asymptomatic CAS. Methods: we selected 15 patients with stroke (group-S) on the basis of the following criteria of inclusion i) stroke onset within the preceding 12 hours ii) carotid artery stenosis iii) no cardiac causes of stroke. We further Abstracts / Atherosclerosis 252 (2016) e1ee196 e176