J Food Biochem. 2020;00:e13381. wileyonlinelibrary.com/journal/jfbc | 1 of 12 https://doi.org/10.1111/jfc.13381 © 2020 Wiley Periodicals LLC Received: 1 May 2020 | Revised: 24 June 2020 | Accepted: 25 June 2020 DOI: 10.1111/jfbc.13381 FULL ARTICLE Antioxidant and antiglycation activities of traditional plants and identification of bioactive compounds from extracts of Hordeum vulgare by LC–MS and GC–MS Awais Asif 1,2 | Nadia Zeeshan 2 | Sajid Mehmood 3 1 Department of Biochemistry, Nawaz Sharif Medical College, University of Gujrat, Gujrat, Pakistan 2 Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakistan 3 Department of Biochemistry, Islam Medical and Dental College, Sialkot, Pakistan Correspondence Nadia Zeeshan, Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakistan. Email: nadia.zeeshan@uog.edu.pk Funding information The authors appreciate the financial support of Department of Biochemistry, Nawaz Sharif Medical College, Department of Biochemistry and Biotechnology, University of Gujrat, Gujrat, Pakistan. They funded to perform the research with the facilitation of IRSIP HEC Pakistan. Abstract Glycation has been involved in Schiff base reaction lead to hyperglycemia at cellular level. The current study aimed to identify the bioactive compounds from selected folkloric plants for their antiglycation and antioxidant potential. Methanol extracts demonstrated the highest activities, therefore, it was further fractionated using n- hexane, dichloromethane, ethyl acetate, and methanol solvents to isolate the nonpolar compounds from the Hordeum vulgare. Moreover, n-hexane and dichloromethane fractions of H. vulgare demonstrated the best antioxidant (61.58% and 62.89%) and antiglycation activities (72.52% and 61.52%) at 2 mg/ml, respectively. Analytical techniques of LC–MS and GC–MS were employed for identification of bioactive compounds; Biochanin A in dichloromethane (DCM) and Vitamin E in n-hexane fractions. There was a strong correlation between antioxidant and antiglycation activities (r = 0.97 and r = 0.96) of DCM & n-hexane fractions of H. vulgare. Findings of this study established the role of Biochanin A and Vit E from H. vulgare as potent antiglycation agents. Practical applications The results of this study confirmed the potential role of Black Barley has involved in the inhibition of protein glycation, which can be the potential treatment to reduce the complications of Diabetic Patients. The Black Barley has a rich source of identified compounds Biochanin A and Vitamin E. We can use this plant as a staple food in curing the severity of diabetes. The other practical approach is to use this plant as an ingredient of different food products. The extraction of identified bioactive compounds from the plant will be a good and cheap source of the treatment. KEYWORDS advanced glycation end products, antiglycation activity, antioxidant activity, bioactive compound, BSA/glucose, dicloromethane (DCM), n-hexane, non-enzymatic glycosylation, plant extracts