164 Published by Indonesia Journal of Biomedical Science | IJBS 2021; 15(2): 164-168 | doi: 10.15562/ijbs.v15i2.319 ORIGINAL ARTICLE Anti-hyperurisemic Activity of Combination of Beetroot Powder (Beta vulgaris L.) And Allopurinol in Potassium Oxonate-Induced White Rats Anggraini Wulandari 1* , Paramasari Dirgahayu 2 , Budiyanti Wiboworini 3 1 Postgraduate Program of Nutrition Science, Universitas Sebelas Maret, Indonesia 2 Department of Parasitology, Faculty of Medicine, Universitas Sebelas Maret, Indonesia 3 Department of Nutrition, Faculty of Medicine, Universitas Sebelas Maret, Indonesia *Corresponding to: Anggraini Wulandari; Postgraduate Program of Nutrition Science, Universitas Sebelas Maret, Indonesia; awulanpurwanto@gmail.com Indonesia Journal of Biomedical Science (IJBS) 2021, Volume 15, Number 2: 164-168 P-ISSN.2085-4773, E-ISSN.2302-2906 Received: 2021-06-11 Accepted: 2021-11-25 Published: 2021-12-21 Published by Indonesia Journal of Biomedical Science ABSTRACT Introduction: Hyperuricemia is an abnormally high quantity of uric acid in the blood. Allopurinol is a type of xanthine oxidase inhibitor commonly used to lower uric acid levels in the general population. However, large doses of allopurinol can produce harmful efects, one of which being hepatotoxicity. The antioxidant activity of beetroot powder is high, and it has an antihyperuricemic efect. Giving beetroot powder and allopurinol together is intended to minimize allopurinol’s adverse efects and give a positive increase in lowering uric acid levels. This study aims to evaluate the anti hyperuricemia efect of beetroot powder (Beta vulgaris L.) and allopurinol combination in white rats (Rattus novergicus strain Sprague Dawley) induced by potassium oxonate. Methods: This an experimental study with a randomized pre and post-test control group design. In this experiment, twenty rats were placed into four treatment groups; KP = Positive Control (hyperuricemic rats) + standard feed and drink; G1 = hyperuricemic rats + allopurinol 1,8 mg/Kg BW/day); G2 = Hyperuricemic rats + beet powder 1.56 g/Kg BW/day; G3 = hyperuricemic rats + allopurinol 1,8 mg/Kg BW/day and beetroot powder 1,56 g/Kg BW/day. On day 0 and day 28, the amounts of uric acid were measured. Data obtained were analyzed using SPSS version 23 for windows. Results: The mean pre-post changes in uric acid levels in each group were KP = 8.97 ± 0.48 mg/dl, G1 = 2.06 ± 0.15 mg/dl, G2 = 2.24 ± 0.10 mg/dl, and G3 = 2.11 ± 0.86 mg/dl. The mean pre-post changes in MDA levels in each group were KP = 8.14 ± 0.39 nmol/mL, G1 = 2.01 ± 0.38 nmol/mL, G2 = 3.13 ± 0.30 nmol/mL, and G3 = 2.35 ± 0.19 nmol/mL. Conclusion: Beetroot powder and allopurinol combination given for 28 days signifcantly reduced uric acid and MDA levels in potassium oxonate-induced white rats. Keywords: beetroot powder, allopurinol, hyperuricemia Cite this Article: Wulandari, A., Dirgahayu, P., Wiboworini, B. 2021. Anti-hyperurisemic Activity of Combination of Beetroot Powder (Beta vulgaris L.) And Allopurinol in Potassium Oxonate-Induced White Rats. IJBS 15(2): 164-168. DOI: 10.15562/ijbs. v15i2.319 Open access: http://ijbs-udayana.org/ INTRODUCTION In Indonesia, hyperuricemia is estimated to afect 1.7 percent of the population. Tis number can increase with age. 1 According to Riskesdas (2018), Riskesdas common illness afects 45 percent of people aged 55 to 64, 51.9 percent of people aged 65 to 74, and 54.8 percent of people aged 75 and above. 2 Te prevalence of common illness is 7.3 percent based on health worker diagnoses and 24.7 percent based on symptoms. Hyperuricemia is common in men, with a prevalence of 17.7 percent and 5.2 percent in women. Men lack estrogen, the hormone which aids in the excretion of excess uric acid through the urine. Women are more susceptible to hyperuricemia as they age because the menopausal phase decreases the production of the hormone estrogen. 3 In humans, uric acid is the result of purine metabolism. Uric acid is made by the enzyme Xanthine Oxidase (XO), which catalyzes the last two steps of the uric acid conversion, namely the conversion of hypoxanthine to xanthine and the formation of uric acid. 4 Elevated uric acid can induce oxidative stress and ROS (Reactive Oxygen Species) production in vascular endothelial cells. Massive ROS can upregulate IL-6, and TNF-α expression. 5 Uric acid is positively correlated with Malondialdehyde. 6 Malondialdehyde (MDA) is a lipid peroxidation product that may be easily identifed in blood or plasma and is used as an oxidative stress indicator. 7,8 According to a study by Zhou et al. (2018), people with hyperuricemia have greater MDA and lower SOD levels than healthy people. 9 Allopurinol is a XO inhibitor frequently used by the public to lower uric acid levels. Treatment failure is reported in 25-50 percent of patients with hyperuricemia due to a suboptimal response to the drug at the recommended dose, failure to tolerate side efects or contraindications. 10 Te most common side efects of allopurinol are gastrointestinal intolerance and skin rashes. 11 Another treatment and therapy