Galley Proof 29/09/2017; 15:22 File: hab–1-hab329.tex; BOKCTP/ljl p. 1 Human Antibodies -1 (2017) 1–6 1 DOI 10.3233/HAB-170329 IOS Press Role of FSHR rs6165 and ESR2 rs4986938 polymorphisms in ovarian stimulation of Iranian women who underwent assisted reproduction treatment Maryam Kaviani a , Sayyed Mohammad Hossein Ghaderian b,∗ , Soheila Arefi c , Mehrdad Hashemi a and Sarah Sadat Aghabozorg Afjeh d a Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran b Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran c GIVAR IVF Center, Erfan Hospital, Tehran, Iran d Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran Abstract. BACKGROUND: Gonadotropin therapy was used to stimulate the ovary in infertile women who underwent assisted reproduc- tion treatment (ART). Numerous studies indicated that infertile women showed different responses to gonadotropin therapy. Fol- licle stimulating hormone receptor (FSHR) and Oestrogen receptor (ER) polymorphisms have been reported to involve induction of folliculogenesis and ovarian response to treatment. METHODS: In the present study, two polymorphisms, namely FSHR rs6165 and ESR2 rs4986938, were investigated in 198 Ira- nian infertile women aged less than 39 years who underwent ART. After DNA extraction, these polymorphisms were genotyped by TaqMan genotyping assay. According to the number of eggs released during ovulation, the patients were categorized into poor responders (PR) and good responders (GR). The results indicated that the good responders showed lower levels of FSH and LH than poor responders. Results: No association was observed between ESR2 rs4986938 and poor response in Iranian women patients. Instead, FSHR rs6165 showed a strong association with ovarian response to ART (p< 0.05). CONCLUSION: The obtained results indicated that FSHR rs6165, not ESR2 rs4986938, could be suggested as a candidate marker to predict poor ovarian response. Keywords: FSHR, ESR2, polymorphism, TaqMan genotyping assay, poor responders 1. Introduction 1 The most widely practised assisted reproductive 2 techniques is in vitro fertilization (IVF). This approach 3 is known to be an effective and safe tool in the treat- 4 ment of infertility. It has opened a new window of 5 * Corresponding author: Sayyed Mohammad Hossein Ghade- rian, Urogenital Stem Cell Research Center, Shahid Beheshti Uni- versity of Medical Sciences, Tehran, Iran. E-mail: Mohammad. taheri@sbmu.ac.ir. hope for infertile couples. The most important indi- 6 cations of IVF included male factor infertility, tubal 7 factor infertility, endometriosis, ovulation disorders, 8 unexplained infertility, ovarian failure,and diminished 9 ovarian reserve (DOR) [1].The success rate is directly 10 correlated with the number of oocytes retrieved, and, 11 in turn, it affected the number of embryos available 12 to transfer. Furthermore, the success of IVF treatment 13 largely depends on controlled ovarian hyperstimula- 14 tion (COH). This technique involved using injection 15 of exogenous gonadotropins to induce multiple folli- 16 ISSN 1093-2607/17/$35.00 c  2017 – IOS Press and the authors. All rights reserved uncorrected proof version