HISTOCHEMICAL STUDIES ON INTESTINAL LYMPHOID TISSUES IN KADAKNATH BREED OF POULTRY (Gallus gallus domesticus) Dr. Prashant Gedam 1 , Dr. Amol Salankar 2 , Dr. Naresh Nandeshwar 3 , Dr. U.P. Mainde 4 , Dr. T. Rama 5 , Dr. Rasmita Panda 6 , Dr. V. Ravikanth 7 and Dr. Sandip Bansode 8 1,2,34 Nagpur Veterinary College, Maharashtra, Nagpur. 5,6,7,8 College of Veterinary Science, Korutla E-mail: nvc1828@rediffmail.com Abstract: The histochemical studies on lymphoid tissue of the intestine was carried out on 30 birds of Kadaknath breed of chicken, divided in to 3 different age groups as GI (6 weeks), GII (12 weeks) and GIII (18 weeks). The lamina propria of the small intestine showed PAS positive material with varying intensity in the different cells. The lymphoid tissue was scattered throughout the intestine in the diffuse and aggregated form in all the groups under present study. The cytoplasm of the epithelium, mucobacterial layer and lamina propria showed the moderate acid mucopolysaccharides in almost all the parts of intestine. The aggregated solitary lymphatic nodules were present in the caeco-colicphatic tissue was predominant in Gr.II birds. The germinal centers were well market in Gr. II and Gr. III birds. The acid phosphatase activity was weak in the lymphoid follicles of Gr.I, but its intensity was more in Gr.III birds. The glycogen activity was strong in lymphatic of Gr. II birds. The neutral mucopolysaccharide was dominant. The acid phosphatase activity was weak where as the alkaline phosphatase activity was stronger in 12-18 week old birds. Keywords: Intestinal lymphoid tissue, kadknath, Peyers patches, Histochemical. 1. Introduction India is ranking currently fifth in the world as a broiler producer and fourth in the egg production. The annual egg production in India is recorded as 53,000 million and poultry meat as 3.2 million tones (Saxena, 2009). Kadaknath breed of poultry is reared since long time by tribals of Bhil and Bhilala community of Jhabua and Dhar districts of Western Madhya Pradesh. The black coloration of the flesh is due to the deposition of melanin pigment in the connective tissue of organs and in the dermis (Rao and Thomas, 1984). The lymphoid tissue of the gut has a significant role in preventing diseases caused by gut pathogens (Hanger and Heath, 1994). It is possible that this tissue respond to antigen, gaining International Journal of Science, Environment ISSN 2278-3687 (O) and Technology, Vol. 6, No 3, 2017, 2132 – 2136 2277-663X (P) Received May 26, 2017 * Published June 2, 2017 * www.ijset.net