1 SUPPLEMENTARY MATERIAL New Chemical constituent from the stem of Cuscuta reflexa Roxb. and its biological activities Zulfa Nooreen, 1 Sudeep Tandon, 1 Narayan Prasad Yadav, 2 and Ateeque Ahmad 1* 1 Process Chemistry and Technology Department, Central Institute of Medicinal and Aromatic Plants, Lucknow-226015, India 2 Herbal Medicinal Product Department, Central Institute of Medicinal and Aromatic Plants Lucknow-226015, India. *Corressponding author. E-mail address: ateeque97@gmail.com ; a.ahmad@cimap.res.in (A. Ahmad) ABSTRACT Chemical investigations on the stem of Cuscuta reflexa Roxb. (Convolvulaceae) led to the isolation of one new compound characterised as 3', 4'-dimethoxy-1-phenyl-1α, 2- ethanediol (1), along with eight known compounds as tridecanyl palmitate, palmitic acid, n- pentatriacontane, n-triacont-21, 27-dien-1-ol, kaempherol, chlorogenic acid, 5, 7- dimethoxyapigenin and quercitin. The chemical structures were established with the help of physical, chemical and spectroscopic methods. The antimicrobial potential of the new compound (1) was evaluated against three bacterial and three fungal pathogenic strain and showed significant activities. KEYWORDS: Cuscuta reflexa; Convolvulaceae; new compound; spectroscopic analysis; antimicrobial activity.