Electronic Supplementry Information (ESI) Zinc (II) Directed Triple-Stranded Helicate Incorporating Nine Membered Metallamacrocycle: Supramolecular Cylinders Mimicking P1 Nuclease Rakesh K. Mishra, a K. K. Upadhyay,* a Sachin Shukla, b R. Mishra b a Department of Chemistry, Faculty of Science, Banaras Hindu University,Varanasi-221005, India. E-mail: drkaushalbhu@yahoo.co.in; kku@bhu.ac.in, b Department of Zoology, Faculty of Science, Banaras Hindu University,Varanasi-221005, India. CONTENTS: 1. Synthetic scheme for complex----------------------------------------------------------------S1 2. Synthesis and Characterization of Ligand---------------------------------------------------S2 3. Synthesis and Characterization of Complex------------------------------------------------S3 4. 1 H NMR spectrum of Ligand----------------------------------------------------------------S4 5. 13 C NMR spectrum of Ligand----------------------------------------------------------------S5 6. LC-MS spectrum of Ligand-------------------------------------------------------------------S6 7. 1 H NMR spectrum of complex---------------------------------------------------------------S7 8. 13 C NMR spectrum of complex---------------------------------------------------------------S8 9. 1 H– 1 H Cosy spectrum of complex-----------------------------------------------------------S9 10. MALDI-MS spectrum of complex---------------------------------------------------------S10 11. Scanning Electron Microscopic (SEM) studies-------------------------------------------S11 12. Crystal refinement parameter---------------------------------------------------------------S12 12.(a). Important crystal data and ortep plot for the ligand ------------------------S12 (a) 12.(b). Table 11 b. Important Bond Length and Bond Angle----------------------S12 (b) 13. Crystal packing for the complex and figure showing hydrogen bonding-------------S13 14. UV-vis Experiment---------------------------------------------------------------------------S14 15. Fluorescent Experiment----------------------------------------------------------------------S15 16. Circular Dichroism Studies-----------------------------------------------------------------S16 17. Agarose Gel Electrophoresis Experiment: ------------------------------------------------S17 17. (a).Plasmid DNA-------------------------------------------------------------------S17 (a) 17. (b).DNA cleavage experiments----------------------------------------------------S17 (b) 18. DNA cleavage experiment for Ligand-----------------------------------------------------S18 Electronic Supplementary Material (ESI) for Chemical Communications This journal is © The Royal Society of Chemistry 2012