~ 85 ~ International Journal of Veterinary Sciences and Animal Husbandry 2023; 8(5): 85-87 ISSN: 2456-2912 VET 2023; 8(5): 85-87 © 2023 VET www.veterinarypaper.com Received: 04-07-2023 Accepted: 03-08-2023 Gorre Venu Ph.D. Scholar, Department of Veterinary Microbiology, ICAR- IVRI, Uttar Pradesh, India S Vamshi Krishna Assistant Professor, Department of Veterinary Microbiology, College of Veterinary Science, PV Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India J Shiva Jyothi Assistant Professor, Department of Veterinary Microbiology, College of Veterinary Science, PV Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India Corresponding Author: S Vamshi Krishna Assistant Professor, Department of Veterinary Microbiology, College of Veterinary Science, PV Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, Telangana, India Antibiogram study of Pasteurella multocida strains isolated from pneumonic sheep and goats in Warangal region of Telangana Gorre Venu, S Vamshi Krishna and J Shiva Jyothi DOI: https://doi.org/10.22271/veterinary.2023.v8.i5b.692 Abstract Pasteurella multocida causing pneumonic pasteurellosis is one of the most economically important infectious diseases of small ruminants having a high prevalence rate and worldwide in distribution. A total of 16 isolates were obtained from the 191 pneumonic sheep and goat. Antibiotic susceptibility testing was performed using 12 antibiotics by the disc diffusion method. In this study, we found that the P. multocida isolates were found 100% sensitive to ceftriaxone, 93.75% for cotrimoxazole and enrofloxicin, 87.5% for amoxicillin-clavulanic acid, cefotaxime and tetracycline, 81.25% for amoxicillin/sulbactum, 75% for chloramphenicol and oxacillin, 68% for gentamicin and Co-trimaxazole, 62.5% for erythromycin and 60% for Sulphadiazine. Emergence of drug resistance in the isolates warrants the judicious use of antibiotics after performing antimicrobial susceptibility testing, wherever possible. Keywords: Antibiotic sensitivity, Pasteurella multocida, Sheep and goats, Telangana 1. Introduction Pasteurella multocida is a Gram-negative bacterium belonging to the family Pasteurellaceae. Different capsular types are associated with wide variety of diseases in animals and birds [1] . P. multocida type A is known to cause pneumonic pasteurellosis in small ruminants. However, other capsular types have been frequently isolated from cases of pneumonia in sheep and goat [2] posing major problem in disease control and prevention. Although antibiotics were originally used to treat pasteurellosis, their repeated and indiscriminate usage has resulted in the development of resistance in a number of strains. Currently, antimicrobial resistance is a global concern, therapeutic alternatives might be difficult, and understanding antimicrobial susceptibility is crucial when applying rationale efficient therapy [3] . Due to scarcity of antimicrobial resistance data on P. multocida, the present study was undertaken to study the antimicrobial susceptibility of P. multocida isolated from sheep and goat in the Warangal region of Telangana state. 2. Materials and Methods A total of 16 P. multocida isolates recovered from 191 samples collected from pneumonic sheep and goat and previously confirmed as P. multocida by cultural, biochemical and molecular testing were included in the study [4] . Bauer-Kirby [5] used a modified disc diffusion method to conduct an antibiotic susceptibility test using antibiotic test discs. Test culture of P. multocida enriched in BHI broth and incubated at 37 ℃ for 7-8 h. The turbidity of the culture was adjusted to McFarland standard tube 0.5 before charging the sheep blood agar plate. Inoculum was spread with the help of sterile cotton swab evenly over entire surface of the sheep blood agar plates by swabbing back and forth across the agar. Antibiotic discs were kept aseptically by using sterile forceps. The plates were then incubated at 37 ℃ for 16-24 h. The zones of growth inhibition around the antibiotic discs were measured to the nearest millimetre. The zone diameter of each drug were interpreted using the criteria chart provided [6] .