_____________________________________________________________________________________________________ *Corresponding author: E-mail: drhemaprakash@gmail.com; International Journal of TROPICAL DISEASE & Health 30(4): 1-11, 2018; Article no.IJTDH.41856 ISSN: 2278–1005, NLM ID: 101632866 Detection of Carbapenem and Colistin Resistant Gram-negative Bacteria in a Tertiary Care Hospital, Visakhapatnam, India Alugupalli Amar Sahas 1 , Payala Vijayalakshmi 1 , Pilli Hema Prakash Kumari 1* , Swapna Sasapu 1 , Ratna Harika Dusi 1 and R. V. Manasa 2 1 Department of Microbiology, GITAM Institute of Medical Sciences and Research, GITAM University, Visakhapatnam-530045 (A.P), India. 2 Department of Community Medicine, GITAM Institute of Medical Sciences and Research, GITAM University, Visakhapatnam-530045 (A.P), India. Authors’ contributions This work was carried out in collaboration between all authors. Author AAS performed the bench work and wrote the first draft of the manuscript. Author PHPK designed the study and wrote the protocol. Author PVL managed the analyses of the study. Authors SS and RHD managed the literature searches. Author RVM performed the statistical analysis of the study.All authors read and approved the final manuscript. Article Information DOI: 10.9734/IJTDH/2018/41856 Editor(s): (1) Zhiheng Zhou, Thyroid Cancer Research Laboratory, Massachusetts General Hospital, Harvard Medical School, Boston, USA. Reviewers: (1) Gokben Ozbey, Firat University, Turkey. (2) Anthony N. Umo, University of Uyo, Nigeria. Complete Peer review History: http://www.sciencedomain.org/review-history/24887 Received 18 th March 2018 Accepted 25 th May 2018 Published 30 th May 2018 ABSTRACT Aims: Among the most clinically significant multidrug-resistant bacteria are the carbapenemase- producing Enterobacteriaceae (CPE) which are detected all over the globe, with a marked endemicity according to enzyme type. These bacteria usually remain susceptible to polymyxins like colistin; however increasing use of colistin causes acquired colistin resistance which may now be added to the carbapenem resistance trait in Enterobacteriaceae. The present study was aimed to isolate and identify Gram-negative Enterobacteriaceae organisms from various clinical samples of Original Research Article