Volume 2 • Issue 5 • 1000e109
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Kolathuru and Yeung, Nat Prod Chem Res 2014, 2:5
DOI: 10.4172/2329-6836.1000e109
Editorial Open Access
Natural Health Products as Modulators of Adenosine and ATP Metabolism
for Cardiovascular Protection
Shyam Sundar Kolathuru and Pollen K Yeung*
College of Pharmacy and Department of Medicine, Dalhousie University, Halifax, NS, Canada
*Corresponding author: Pollen K Yeung, Dalhousie University, Halifax, NS,
Canada, Tel: 902-4943845; Fax: 902-4941396; E-mail: Pollen.Yeung@Dal.Ca
Received July 18, 2014; Accepted July 20, 2014; Published July 22, 2014
Citation: Kolathuru SS, Yeung PK (2014) Natural Health Products as Modulators
of Adenosine and ATP Metabolism for Cardiovascular Protection. Nat Prod Chem
Res 2: e109. doi:10.4172/2329-6836.1000e109
Copyright: © 2014 Kolathuru SS, et al. This is an open-access article distributed
under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the
original author and source are credited.
Introduction
Adenosine is an important endogenous purine nucleoside and
an essential component of the molecular energy generated from
adenosine 5’-triphosphate (ATP). It acts as both a precursor and
metabolite of adenine nucleotides. As every cell utilizes the energy
generated from catabolism of ATP, adenosine is found ubiquitously in
the body. It is also a signaling molecule in the cardiovascular system,
and its role for cardioprotection and cardiovascular homeostasis has
been studied for over 80 years [1-3]. Adenosine is also known as a
“homeostatic metabolite in cardiac energy metabolism” [4] owing to
its wide range of benefcial efects on the cardiovascular system [5]
which include negative chronotropic and dromotropic efect in cardiac
tissue, vasodilatation, inhibition of platelet aggregation, modulation of
vascular smooth muscles and endothelium and induction of ischemic
preconditioning [6]. Adenosine modulates these actions by interacting
with adenosine receptors (AR), which are widely distributed throughout
the body. It is believed that metabolic condition of the myocardium
may be assessed quantitatively by the level of adenosine production
which maintains a healthy balance between energy supply and demand
in the cardiovascular system [7].
Under adverse conditions such as ischemia, hypoxia, trauma,
seizure, infammation and painful stress, there is an increased demand
for energy which is met by the increased catabolism of ATP to AMP
and subsequently adenosine resulting in elevated levels of adenosine in
both extracellular and intracellular space [8,9]. Te increased adenosine
concentration ensures protection against further tissue damage or
organ dysfunction [10]. Tere have been many studies both in animal
models and humans to explore the cardiovascular efects of adenosine
in the past few decades. Tese studies have laid the ground work and
set the pace for future research on adenosine, adenosine agonists and
adenosine re-uptake inhibitors as cardiovascular protective agents.
Adenosine Production and Metabolism Under
Physiological Conditions in the Cardiovascular System
Under normal physiological conditions the main source of
adenosine in intracellular and extracellular space is from catabolism
of ATP to ADP and then to AMP, which is further catabolized by ecto
and endo 5`nucleotidase to produce adenosine [11]. Another source of
adenosine is from hydrolysis of S-adenosylhomocystein which is derived
from the transmethylation pathway from S-adenosylmethionine [12].
Intracellular adenosine can undergo rephosphorylation to form AMP
and other adenine nucleotides by adenosine kinase [13] or metabolized
to inosine by adenosine deaminase respectively [14], which maintain
low concentrations of adenosine under basal (normal) condition [11].
However, during ischemia/hypoxia or in extremely heavy workloads,
there is an increased demand of energy such that an imbalance between
energy supply and demand may occur which increases ATP breakdown
to produce other high energy phosphates (e.g. ADP and AMP) [15].
Tis can cause an increase in adenosine production in the myocytes,
vascular endothelium, smooth muscle cells [16] and in the RBC [17].
Te adenosine released into the circulation is taken up rapidly by
endothelial cells and red blood cells via nucleoside transporters and
subsequently metabolized [18,19].
Adenosine Receptors and Cardiovascular Protection
Adenosine is a key mediator in ischemia preconditioning which
is an important factor responsible for cardiovascular protection [20].
Adenosine modulates its actions via membrane bound adenosine
receptors which are coupled to G-protein and subdivided into 4
diferent subtypes: A
1
, A
2a
, A
2b
and A
3
[21-23]. Cross-talks between
the receptors are known to occur which self regulates and provokes a
specifc cardiovascular response. For example activation of A
1
receptor
induces vasoconstriction which counteracts the A
2
mediated dilating
efect on vascular tone [24]. Similarly, stimulation of the A
2
receptor
increases cardiac contractility which is attenuated by the response
mediated via the A
1
subtype [25]. On the other hand, it was suggested
that cardioprotection from adenosine against myocardial infarction
may be mediated via stimulation of the A
1
receptor [26]. Further, there
is evidence to suggest adenosine receptors may also interact with the
opioid receptors which together protect cellular damage and cell death
caused by ischemia-reperfusion injury [27].
Several lines of experimental studies have shown
that adenosine plays a key role in reactive hyperemia [28] and
cardioprotection by increasing coronary blood fow and attenuating
the breakdown of ATP in myocardium during ischemia and facilitating
its repletion when perfusion is restored during recovery [29-31].
However due to the rapid cellular uptake into RBC, myocardium
and endothelium by nucleoside transporters, pharmacological efects
of adenosine are extremely short lived (<1 min) [19,32]. Several
therapeutic strategies have been explored to prolong the action of
adenosine. Intra-coronary infusion of adenosine was shown to ofer
cardioprotection in a rabbit model of ischemia and reperfusion [33],
but clinical application of this approach is restricted and not practical in
most clinical settings. Another approach of exploiting adenosine is use
of adenosine agonists which is much more stable and longer acting, but
fnding a suitable adenosine receptor agonist with optimum safety and
efcacy profle for a specifc cardiovascular disease condition remains
a therapeutic challenge [2]. A further therapeutic strategy is to inhibit
adenosine uptake by the transporter to exploit the benefcial properties
of adenosine in the cardiovascular system [34]. Administration of
exogenous adenosine in the presence of an inhibitor of the transporter
such as calcium channel blockers or dipyridamole has been shown to
enhance the action of adenosine in vivo in animal models as well as
in patients [35-37]. Tese approaches may have important therapeutic
implications and warrant further investigation.
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ISSN: 2329-6836
Natural Products Chemistry & Research