ELEKOFEHINTI, Olusola Olalekan et al. /Afr.J.Bio.Sc. 4(4) (2022) 56-61 Page 56 of 61 Volume 4, Issue 4, October 2022 Received : 04 March 2022 Accepted : 12 September 2022 Published : 05 October 2022 doi: 10.33472/AFJBS.4.4.2022.56-61 Article Info © 2022 ELEKOFEHINTI, Olusola Olalekan et al. This is an open access article under the CC BY license (https:/ / creativecommons.org/ licenses/ by/ 4.0/ ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. A bstract The study evaluated the effect of Saponins from Tithonia diversifolia leaf on lipid profile of normal rats. 35 rats were divided into six groups of five animals each. Group 1 served as control while groups 2-6 were administered 20, 40, 60, 80 and 100 mg/ kg of Saponins from Tithonia diversifolia leaf for 21 days. Results indicated that administration of Saponins significantly reduced (p<0.05). Triglyceride (TG), Total Cholesterol (TC) and Low Density Lipoprotein (LDL) and increased the level of High Density Lipoprotein (HDL). These results suggest that Saponins from T. diversifolia leaf can prevent serum TG and cholesterol which are risk factors of coronary heart disease. Further studies at the molecular level are needed to understand the mechanism. Keywords: Tithonia diversifolia, Lipid profile, Saponin, Thin Layer Chromatography (TLC), A therosclerosis 1. Introduction Hyperlipidemia has been ranked as one of the greatest risk factors contributing to the prevalence and severity of coronary heart diseases ( Grundy,1987; and Elekofehinti et al., 2012), stroke, atherosclerosis and hyperlipidemia are the primary cause of death (Davey, 1993; and Elekofehiti, 2012). It has been proven to be a contributory cause of atherosclerosis in patients with diabetes (Pearl et al., 1987; Bierman,1992; and Keaney and Localzo, 1999). Excessive accumulation of fatty acids leads to an accumulation of triglyceride in many tissues, particularly in the fat tissue, inducing lipolysis. The important role of serum lipid in atherosclerosis * Corresponding author: ADU, Olumuyiwa Mark, Federal College of Veterinary and Medical Laboratory Technology Vom, Plateau State, Nigeria. E-mail: adu2blossom@live.com 2663-2187/ © 2022. ELEKOFEHINTI, Olusola Olalekan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. African Journal of Biological Sciences ISSN: 2663-2187 Journal homepage: http:/ / www.afjbs.com Effect of saponin from Tithonia diversifolia leaf on the lipid profile of normal albino rats ELEKOFEHINTI, Olusola Olalekan 1 , ADU, Olumuyiwa Mark 2,4 , GOTEP, Jurbe Gofwan 3 , RAHEEM, Kayode Yomi 4 , OLANREWAJU, Ojuolape Damilola 5 , OYEKAN, Olumuyiwa Adediran 6 , OGUNMOLAWA , Oluyemi Rotimi 7 , UJAH, Francisca Eborh 8 and YAHAYA, Dorcas Iye 9 1 Department of Biochemistry, Federal University of Technology Akure, Ondo State, Nigeria. E-mail: ooelekofehinti@futa.edu.ng 2 Federal College of Veterinary and Medical Laboratory Technology Vom, Plateau State, Nigeria. E-mail: adu2blossom@live.com 3 Drug Development Division, National Veterinary Research Institute, Vom, Plateau State, Nigeria. E-mail: jurbegotep@gmail.com 4 Adekunle Ajasin University Akungba Akoko, Ondo State Nigeria. E-mail: kayoderaheem.y@gmail.com 5 Federal College of Animal Health and Production Technology Vom, Jos, Plateau State, Nigeria. E-mail: ojuola04@gmail.com 6 National Veterinary Research Institute (NVRI) Vom, Jos, Plateau State, Nigeria. E-mail: olumuyiwaoyeks@gmail.com 7 National Veterinary Research Institute (NVRI) Vom, Jos, Plateau State, Nigeria. E-mail: yemioguns4u@gmail.com 8 University of Jos, Plateau State, Nigeria. E-mail: fransciscaujah5@gmail.com 9 Federal College of Veterinary and Medical Laboratory Technology Vom, Plateau State, Nigeria. E-mail: dorcasyahayaiye@gmail.com Research Paper Open Access ELEKOFEHINTI, Olusola Olalekan et al. /Afr.J.Bio.Sc. 4(4) (2022) 56-61 https://doi.org/10.33472/AFJBS.4.4.2022.56-61