Research Article A Physical-Chemical Study of the Interference of Ceftriaxone Antibiotic with Copper Chloride Salt Elsayed M. AbouElleef , 1,2 Mowafak M. Mahrouka, 1 and Sherine E. Salem 3 1 Chemistry Department, Faculty of Arts and Science, Rafha Northern Border University, 91911 Rafha, Saudi Arabia 2 Basic Science Department, Delta Higher Institute for Engineering and Technology, Mansoura,, 35681 Dakhlia, Egypt 3 Chemistry Department, Faculty of Science, Mansoura University, 35516 Mansoura, Egypt Correspondence should be addressed to Elsayed M. AbouElleef; s.abouelleef@yahoo.com Received 7 August 2021; Revised 2 October 2021; Accepted 6 October 2021; Published 19 October 2021 Academic Editor: Guillermo Mendoza-Diaz Copyright © 2021 Elsayed M. AbouElleef et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. e nano-CuCl 2 .2H 2 O salt was prepared by the ball milling method. e association parameters for bulk and nano-CuCl 2 salts in H 2 O are estimated at different temperatures using the conductivity method by applying the Fuoss–Shedlovsky equation and it was higher for nano-CuCl 2 than bulk CuCl 2 salt. e interaction between the cation (Cu 2+ ) and ligand (ceftriaxone) in H 2 O was determined also by the conductometric method. Two stoichiometric complexes 1/2 and 1/1 (M/L) are estimated and follow the order K f (1/1) > K f (1:2) and G f (1/1) > G f (1/2) for (M:L) (in negative values) indicate the favorable of formation of (1/1) complex compared to the (1:2) complex. e Gibbs free energies change was increased in negative signs with increasing the temperature. e antimicrobial activities of CFT, bulk Cu-CFTcomplex, and nano-Cu-CFT complex were studied on LB agar by the disc diffusion technique against clinical isolates of gram-negative bacteria (Klebsiella pneumonia and Pseudomonas aeruginosa) and Fungi (Candida albicans). It was observed that (CFT) has a higher zone of inhibition and antibacterial activity than that of bulk and nano-Cu-CFTcomplexes in Klebsiella pneumonia and Pseudomonas aeruginosa (gram-negative bacteria). e nano-Cu-CFT complex showed a higher clear zone of inhibition and antifungal activity against candida than bulk Cu-CFT complex while the absence of the inhibition zone in CFT, so nano-Cu-CFT complex, can be used as an antifungal drug. 1. Introduction Nanoparticles (NPs) are a wide class of materials that have a state between bulk and atomic or molecular structures in different shapes of 0D, 1D, 2D, or 3D and have a great scientific interest [1–5]. Bulk materials have constant physical properties, with size larger than one micrometer or micron. Nanoparticles can be used for various applications such as drug delivery purposes [6], diagnostics of cancer therapy, gene delivery purposes, chemical and biological sensing [7], gas sensing [8–10], CO 2 capturing [11, 12], and other related applications [13–17]. Nanoparticles are often used to photocatalytically break down oil into biodegradable compounds, break down volatile organic pollutants in the air, and clean up carbon tetrachloride pollution in spring water [18, 19]. Nanoparticles (NPs) such as gold, silver, platinum, and palladium showed colors with the variation of shape and size and characteristic properties that can be utilized in bioimaging applications [20]. Another applica- tion of nanoparticles is the synthesis of photocatalysis S-doped TiO 2 nanoparticles and the study of their photo- catalytic, antimicrobial, and antioxidant activities under sunlight illumination [21]. Ceftriaxone [22–25] is an antibiotic that belongs to a category of medicine referred to as cephalosporin anti- biotics, and it treats a variety of bacterial infections by stopping the growth of bacteria variety of bacterial in- fections (e.g., middle ear, lower tract, skin, and urinary tract), meningitis, gonorrhea, pelvic disease, and joint infections. Hindawi Bioinorganic Chemistry and Applications Volume 2021, Article ID 4018843, 15 pages https://doi.org/10.1155/2021/4018843