Downloaded from http://journals.lww.com/acsm-msse by BhDMf5ePHKbH4TTImqenVKrtzFmpNzJo8DS/3GvHyfPaNSZtdVgDUi8IAQ+8pBp4 on 09/16/2020 465 1929 Board #190 May 31 2:00 PM - 3:30 PM MCT1 Gene Function on Percentage Fat Responses in Overweight and Obese Humans Rocío Cupeiro 1 , Barbara Szendrei 1 , Teresa Amigo 2 , Pedro J. Benito 1 , Domingo González-Lamuño 2 . 1 Universidad Politécnica de Madrid, Madrid, Spain. 2 University of Cantabria/ IDIVAL, Santander, Spain. (No relevant relationships reported) Transgenic mouse for the Monocarboxylate Transporter 1 (MCT1) gene (SLC16A1) with one invalidated allele presented resistance to diet-induced obesity. In humans a single-nucleotide polymorphism (SNP), T1470A (rs1049434), for this gene has been related with an impaired lactate transport in T male carriers. Therefore, this SNP could have an influence in body composition changes, simulating the results seen in transgenic mice. PURPOSE: To investigate the influence of the T1470A SNP of the MCT1 gene on percentage fat (%fat) changes after a 6-month weight loss program in obese and overweight healthy people. METHODS: 91 women (39.1±8.3 years, 80.7±10.5 kg) and 84 men (39.6±8.2 years, 96± 10.7 kg) followed a 24-week weight loss intervention including a controlled training program (supervised exercise group, S: 3 times/week, 38-60 min/session; strength, endurance and combined training; N=134) or exercise recommendations (non-supervised exercise group, NS; N=39). All groups had caloric restriction of 25-30% of their energy expenditure. Genotyping was done using PCR and direct sequencing or Real Time PCR. Three-way (genotype x exercise group x sex) ANCOVA was conducted for compare the final values of %fat, adjusting by the initial values. Effects sizes (ES) and their 90% confidence intervals (CI) were calculated to show the magnitude of the effect (standardized differences in means: Cohen’s units) of carrying the A allele. RESULTS: A genotype x exercise group x sex interaction was observed (p=0.017). TT women had less final %fat in the whole group (p=0.025, 1.94%) as well as within the NS group (p=0.002, 4.49%) than A carriers. The ES associated indicate that the A allele has a moderate (ES=1.15, CI=1.88, 0.42) and very likely positive effect only in women within the NS group. For men the A carriers ended with less %fat than TT subjects (p=0.037, 1.87%), with small and possibly negative ES in the S group (ES=-0.25, CI=-0.02, 0.52) and small and likely negative in NS (ES=-0.46, CI=0.01, 0.91). CONCLUSIONS: The T1470A SNP might have an influence on %fat changes after a weight loss program. The effect seems to be different depending on sex and type of exercise intervention. Further studies are necessary to confirm this association and to clarify the underlying mechanisms. Supported by Spanish Government Grant DEP2008-06354-C04-01. 1930 Board #191 May 31 2:00 PM - 3:30 PM Effects Of Exhaustive Exercise On PHB1 Expression And Mitochondrial Function In Rats Hong Feng 1 , Ze Li 1 , Wen Fang 2 , Zhaoming Liu 1 , LiLi Ji, FACSM 3 . 1 Tianjin Unversity of Sport, Tianjin, China. 2 Tsinghua Unversity, Beijing, China. 3 University of Minnesota, Minneapolis, MN. (Sponsor: Li Li Ji, FACSM) (No relevant relationships reported) F0F1-ATP synthase is the key enzyme of mitochondrial oxydative phosphorylation, which largely determines the exercise endurance. PHB1 participates in mitochondrial oxidative phosphorylation. The content of PHB1 predicts that of F0F1-ATP synthase in many biological conditions. PURPOSE: To observe the changes of PHB1 content in mitochondria and its relationship with mitochondrial function and energy metabolism in response to an acute bout of exhaustive exercise. METHODS: Male Sprague-Dawley rats were subjected to an acute bout of exhaustive exercise on treadmill at 20 m/min, at which 0% grade. Mitochondria of heart, brain and gastrocnemius were isolated to detect the changes of RCR and ROS. The content of PHB1 protein in mitochondria was detected by Western blot. ATP content in the organs and F0F1- ATP synthase activity were measured by spectrophotometer. RESULTS: 1. Compared with resting control group, ATP content decreased in the brain(-76%, p<0.05), heart(-77%, p<0.01) and skeletal muscle (-55%, p<0.05) after exercise. 2. F0F1- ATP synthase activity declined significantly in the brain(-26%, p<0.05), heart(-58%, p<0.01) and skeletal muscle(-55%, p<0.01) after exercise. 3. Mitochondrial respiratory control ratio (RCR ) was reduced in the brain(-52%, p<0.05), heart(-43%, p<0.05) and muscle (-39%, p<0.05) in response to exercise. 4. ROS generation in mitochondria of brain(-64%, p<0.01), heart(-42%, p<0.05) and muscle (-44%, p<0.05) decreased significantly after exhaustive exercise. 5. Exercise decreased PHB1 content in the mitochondria of muscle(-30%, p<0.01) and brain(-37%, p<0.05) but had no effect on PHB1 in the heart. 6. ATP content was positively correlated with PHB1 level in the brain(r=0.836, p<0.05), heart (r=0.909, p<0.05) and muscle(r=0.913, p<0.05) after exercise, whereas F0F1-ATP synthase activity was positively correlated with PHB1 in the brain(r=0.896, p<0.05), heart (r=0.909, p<0.05) and muscle(r=0.955, p<o.05). 7. ROS generation was positively correlated with muscle PHB1 level (r=0.874, p<0.05) but there was no such correlation in the heart or brain. CONCLUSIONS: An acute bout of exhaustive exercise reduced the expression of PHB1 in the mitochondria of organs examined and decreased mitochondrial bioenergetics. Supported by NSFC(No. 31470061). 1931 Board #192 May 31 2:00 PM - 3:30 PM Effects Of A Ketogenic Or A Whey Protein Supplement On Metabolism, Appetite And Energy Intake Isabel L. Smith, Elizabeth K. Bailey, Theresa Ramos, Takudzwa A. Madzima. Elon University, Elon, NC. (Sponsor: Paul C. Miller, FACSM) (No relevant relationships reported) The satiating effects of protein and its role in energy expenditure have been compared to carbohydrates and fats. Less data exists on the effects of a high fat ketogenic supplement consumed as part of a breakfast smoothie on metabolism, ratings of appetite and energy intake when compared to a protein based breakfast smoothie. PURPOSE: To determine the effects of whey protein (WP) vs. a ketogenic supplement (KS), added to a breakfast smoothie on energy expenditure (EE), appetite and energy intake. METHODS: Fifteen women (age, 3011 yrs; body fat 29.3±5.7%) participated in this randomized, double blind, crossover study. After a 12-hr fast, resting oxygen consumption (VO 2 ) and respiratory quotient (RQ) were assessed via indirect calorimetry. Ratings of appetite (hunger (H), fullness (F), desire to eat (DE) and prospective food consumption (PC)) were assessed via visual analog scales (VAS). After consuming the isocaloric (450 kcals) test meal containing either 54g WP (40% kcals protein) or 52g KS (40% kcals fat), VAS on appetite were administered every 30 min for the 3-hr post-prandial period. The thermic effect of the meal (VO 2 ) and RQ were assessed at 45, 105 and 165 min after meal completion. An ad libitum lunch meal was provided to assess subsequent energy intake. Repeated measures ANOVA were used to analyze data. RESULTS: There was a significant group by time effect for VO 2 (p<0.001) and RQ (p=0.001). WP elicited a greater VO 2 compared to KS at 45, 105 and 165 min (WP: 4.05±0.59, 3.86±0.53, 3.57±0.55 vs. KS: 3.66±0.59, 3.43±0.60, 3.26±0.45 ml/kg/min). RQ was significantly lower after WP compared to KS at all three time points (WP: 0.79±0.03, 0.79±0.04, 0.77±0.03 vs. KS: 0.84±0.04, 0.80±0.06, 0.77±0.08). Significant group by time interactions were observed for H (p=0.022), F (p<0.001) and DE (p=0.02) but not PC (p=0.107). Perceived H and DE were significantly lower, and perceived F was greater in the WP condition compared to KS. Energy intake (WP: 578±282 vs. KS: 625±220 kcals) did not differ between conditions (p=0.197). CONCLUSION: WP appeared to have a marked improvement in increasing both fat oxidation and energy expenditure during the post-prandial period. As such, WP would seem to be more advantageous than KS in promoting overall fullness, however it did not elicit lower subsequent energy intake at an ad libitum lunch meal. 1932 Board #193 May 31 2:00 PM - 3:30 PM Changes In 6-Minute Walk Performance Is Associated With Weight Loss Following A 6-Month Weight Loss Program Katherine A. Collins 1 , Sara J. Kovacs 2 , Shawn D. Raybuck 1 , Audrey M. Collins 1 , Marissa L. Marcin 1 , Alexander L. Carne-Clark 1 , Alyssa M. Harris 1 , Gary D. Foster 3 , Alexis Wojtanowski 3 , John M. Jakicic, FACSM 1 , Renee J. Rogers 1 . 1 University of Pittsburgh, Pittsburgh, PA. 2 Temple University, Philidelpha, PA. 3 Weight Watchers International, Inc, New York, NY. (Sponsor: John M. Jakicic, FACSM) (No relevant relationships reported) There is evidence of a relationship between obesity and decrements in mobility and function, making these important targets for weight loss interventions. Within commercial weight loss programs, there is limited data to quantify changes in functional outcomes such as walking performance and whether weight loss contributes to improvements in this important health outcome. Copyright © 2018 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited.