~ 95 ~ International Journal of Herbal Medicine 2016; 4(6): 95-97 E-ISSN: 2321-2187 P-ISSN: 2394-0514 IJHM 2016; 4(6): 95-97 Received: 14-09-2016 Accepted: 15-10-2016 Jitendra Patel Research Scholar, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, TS, India Venkateshwar Reddy Anwarul Uloom College of Pharmacy, New Mallepally, Hyderabad, TS, India GS Kumar Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jallil, Kuala Lumpur, Malaysia D Satyasai KVK College of Pharmacy, Surmaiguda (V), Hayathnagar (M), RR Dist, TS, India Shiva Krishna KVK College of Pharmacy, Surmaiguda (V), Hayathnagar (M), RR Dist, TS, India Correspondence Jitendra Patel Research scholar, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, TS, India GC-MS Analysis of bioactive components on the Leaves extract of Artocarpus hirsutus: A potential folklore medicinal plant Jitendra Patel, Venkateshwar Reddy, GS Kumar, D Satyasai and Shiva Krishna Abstract Background: Artocarpus hirsutus belongs to the family Moraceae and this comprises 50 varieties of species. They are deciduous and evergreen tall tree grows up to 75 meters in height in southern regions of India. Objective: The primary objective of this study was to investigate the bioactive constituents from (MEAH) methanolic extract of Artocarpus hirsutus leaves using (GC-MS) Gas Chromatography and Mass Spectroscopy. Material and methods: The methanolic extract obtained was subjected to GC-MS for the determination of bioactive volatile compounds. GC-MS analysis was carried out using 6890 GC with 5973 I MSD Column. Helium was used as the carrier gas, the temperature programming was set with initial oven temperature at 400C and held for 3 min, and the final temperature of the oven was 4800C with rate at 100C. A 2-μl sample was injected with split less mode. Mass spectra was recorded over 35-650 amu range with electron impact ionization energy 70 eV. The total running time for a sample is 20 min. Results: The GC-MS analysis of the methanolic extract revealed the presence of 18 bioactive compounds with valuable biological activities. The major chemical constituents are Butanal, 3-methyl; 2- Methylbutyraldehyde; 2-Methyl-3-propyloxirane; 2-Furancarboxaldehyde 5-methyl-; Propylphosphonic dichloride; 5-Hydroxymethylfurfural; 3,3-Dimethylbutan-2-yl methylphosphonofluoridate; N- PENTADECANE; 2,6-dimethoxy-4-vinyl phenol; Quinic acid; 5,6-Dimethoxy-2-methyl-1-indanone; and n- Hexadecanoic acid. Conclusion: The presence of various bioactive compounds in A. hirsutus proved that the pharmaceutical importance. It can be concluded that the plants investigation have opened up a new perspective in pharmaceutical research and they can be used for the development of potential, novel antioxidant agents for the treatment of many diseases. Keywords: GC-MS, Phytochemicals, Artocarpus hirsutus, Methanolic extract 1. Introduction Artocarpus hirsutus belongs to the family Moraceae and this comprises 50 varieties of species. They are deciduous and evergreen tall tree grows up to 75 meters in height in southern regions of India. This species occurs wild and is also cultivated for its edible fruits, leaves, bark and also timber. It is known by a variety of names such as Aani, Aini, Aini-maram, Anjili and Anhili found in Karnataka, Kerala and Tamil Nadu. The plant image is shown in Fig.1. The other Artocarpus genus like Artocarpus altilis (bread fruit), Artocarpus heterophyllus (jack fruit) have medicinal value of their source as an edible aggregate fruit [1] . Artocarpus hirsutus (Wild jack fruit) is been used in antimicrobial activity [2] anti-ulcer activity [3] traditional medicine, food and industry [4] . Plants are vital for the remedies as well as existence for human disease because they contain components of therapeutic value [5] . A number of Artocarpus species are used as traditional medicine is South-East Asia; they contain medicinally important secondary metabolites possessing useful biological activities [6] . The medicinal actions of plants are unique to particular plant species [7] . Therefore, it is worthwhile to use modern tools for verifying therapeutic potential of Artocarpus hirsutus. Such information may unravel novel treatment strategies for disorders [8] . Based on the arena of research data and review this study was taken to investigate phytochemical analysis leave extracts present in this plant which is known to have pharmacological importance. Plants are a rich source of secondary metabolites with remarkable biological activities. The secondary metabolites are significant source with a variety of structural arrangements and properties [9] . Natural products which come out from medicinal plants are important for pharmaceutical research and for drug development as a source of therapeutic agents. At presents the demand for herbal or medicinal plant products has increased significantly [10] .