Int.J.Curr.Microbiol.App.Sci (2021) 10(10): 402-410 402 Original Research Article https://doi.org/10.20546/ijcmas.2021.1010.048 Identification of Cryptosporidium sp. with the Microscopic Examination Method on HIV/AIDS Patients in Padang, West Sumatra Nuzulia Irawati 1* , Nora Harminarti 1 , A. K. Munzi Yulianto 1 and Kemala Sayuti 2 1 Departement of Parasitology, Faculty of Medicine, Andalas University, Padang, 25127, Indonesia 2 The eye part of the hospital is in M Djamil Padang, Padang, 25127, Indonesia *Corresponding author ABSTRACT Introduction Cryptosporidiosis is a zoonotic disease belonging to the waterborne disease. This disease is caused by parasites of the genus Cryptosporidium which are pathogenic organisms and are obligate intracellular. Cryptosporidium sp. can cause acute diarrhea International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 10 Number 10 (2021) Journal homepage: http://www.ijcmas.com Cryptosporidiosis is a zoonosis disease which classified as waterborne disease caused by Cryptosporidium sp. and can inflict acute to chronic diarrhea through small intestine infection. The basic in diagnosing cryptosporodiosis is microscopic or moleculer inspection of feces samples. The objective of this study was to find out the Cryptosporidium sp. infection in HIV/AIDS patients with cluster of differentiation 4 (CD4) ≤ 350 sel/mm3 in m djamil padang. This study was observational descriptive research by using feces, questionnaires, and medical record. This research samples were part of the population which fulfill in the inclusion and exclusion criteria in VCT Policlinic of m djamil padang. Two from forty respondences of this study was found positive of Cryptosporidium sp. Oocyst in their feces. The frequencies based on gender could not be determined because the different number of men and women respondences. From the anamnesis by using questionnaires, this two respondences with positive result did not have diarrhea (asymptomatic). Based on data on drinking water sources and daily water sources as a risk for Cryptosporidium sp infection, there were no significant numbers. From the same typed of water sources, more was found to be negative oocyst than positive oocyst. Based on the source of their drinking water, one of the used cooked water and the other one used retail refill water. Based on their daily water resources, both of them using Water Supply Company (PDAM). It could be influenced by the location, environmental factors, and activities of each of them those were different. Keywords Zoonotic disease, Cryptosporidium, waterborne disease, parasites Accepted: 15 September 2021 Available Online: 10 October 2021 Article Info