Corresponding author: Aremu Abdulfatai Email: aremu.a@unilorin.edu.ng Tel: +23(480)6349-1036 Iranian Journal of Veterinary Science and Technology Received: Accepted afer revision: Published online: ABSTRACT Keywords Abbreviations https://IJVST.um.ac.ir DOI: 10.22067/ijvst.2023.80490.1219 RESEARCH ARTICLE Lawsonia inermis possesses a signifcant analgesic ac- tivity compared to Waltheria indica, Moringa oleifera, Nigella sativa, and diclofenac in female Wistar rats Aremu Abdulfatai Idris F. Jiddah, Akorede G. Jimoh, Olatunji O. Aishat, Afsu Basiru, Ahmed O Akeem Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Department of Veterinary Physiology and Biochemistry, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Nigeria. Pain is a severe symptom of many diseases, with an increasing percentage of people manifesting various types of pain. Medicinal plants provide analgesic potential with little toxicity. We performed this experiment to compare the analge- sic activities of Lawsonia inermis, Waltheria indica, Moringa oleifera, and Nigella sativa in Wistar rats using writhing and paw lick responses. We grouped 21 adult female rats into seven groups (n=3), including uninduced and untreated rats (group 1), induced untreated rats (group 2), rats treated by Lawsonia inermis at 200 mg/kg (group 3), rats treated withWaltheria indica at 200 mg/kg (group 4), rats treated with Nigella sativa at 200 mg/kg (group 5), rats treated with Moringa oleifera at 200 mg/kg (group 6), and rats treated with diclofenac at 10 mg/kg (group 7). We dosed rats for 14 days afer inducing the pain. Phytochemical screening showed that methanolic extracts of Lawsonia inermis, Moringa oleifera, and ethanolic extract of Waltheria indica contain: Alkaloid, saponin, steroid, tannin, favonoid, phenols, ter- pene, and glycosides. Te rate of weight gain in rats treated with M. oleifera and W. indica was 7%, and with diclofenac was 9% compared to the untreated control. L. inermis and N. sativa possessed a weight gain of 3% and 2%, respectively. All the extracts exhibited analgesic activities by signifcantly reducing the number of lick and writh in the order of Law- sonia inermis, Nigella sativa, Moringa oleifera, and Waltheria indica.Tis study concluded that Lawsonia inermis possess signifcant analgesic activities compared to other plants and the standard drug (diclofenac). Pain, Lawsonia inermis, Nigella sativa, Moringa oleif- era, Waltheria indica, Analgesic Number of Figures: Number of Tables: Number of References:: 2023- Feb-03 2023- Jul-01 2023- Jun-13 Number of Pages: 2 30 8 5 a c a NSAIDs: Non-steroidal anti-infammatory drugs Cox: cyclooxygenase GCs: Glucocorticoids; L:Lawsonia M: Moringa W: Waltheria N: Nigella a b c b a a