110 Burazor Miscellaneous Conclusions: CR calculation is not widely used to consider LLT in dys- lipemic patients, including diabetics. Better strategies for implementation of guidelines are needed. CLINICAL AND EPIDEMIOLOGICAL CHARACTERISTICS OF ACUTE MYOCARDIAL INFARCTION IN WOMEN OF NIS M. But'azor, I. Burazor, Z. Perisic, N. Karanovic, S. Nagorni. Clinic for Cardiovascular Diseases, Nis, Serbia Atherosclerosis of coronary arteries is clinically manifested as acute my- ocardial infarction (AMI), disease which accounts for the majority of deaths around the world. We wanted to present the disu'ibution of AMI and major characteristic among the women of Nis. We analyzed data of all patients with diagnosis: acute myocardial infarctions who were hospitalized in Coronary Care Unit of Clinic for Cardiovascular Diseases in Nis, since the formation of the Unit in 1974. Since January 1974 till the end of 2002 the number of patients who were hospitalized with diagnosis acute myocardial infarction was 6987. There were 2110 (30.22%) women. Between them 376(17.82%) died. The youngest woman was 18 and the oldest 93 years of age old. The middle age of the women 64.9 We wanted to know in which month AMI is the most fi'equent: in April and May; while in July the number of patients was the lowest. The majority of women were living in the city of Nis, 1504 (71.27%), while 506 women were living on the villages around Nis. Anterior localization of the AMI had 882 (41.8%), inferior 1119 (53.03%) and inferoanterior 85 (4.2%) pts. Since the first fibrinolytic ~eatment was applied, in 1985, the totals of 347 (16.4%) pts were given s~eptokinase. The total of 349 patients had non q wave myocardial infarction. The results of out" study showed that between women with myocardial infarction majority lived in the city. The disu'ibution fi'om 1974 till 2002 increased, mostly after the bombing in 1999. .4~] REPEATABILITY OF BRACHIAL FMD MEASUREMENTS IN HEALTHY ADULTS AND POWER CALCULATION FOR STUDIES J. Butzelaar, R. Duivenvoorden, B. Elsen, R. Draijer, A. van Roon, E. Stroes, J. Kastelein, E. de Groot. Amc Vascular Medicine, Amsterdam, Unilever Health Institute, Vloardingen, Vascular Lab Internal Medicine, Groningen, The Netherlands Objectives: Endothelial function (EF) and hence the release of the en- dogenous vasodilator nitric oxide (NO) is impafl'ed in subjects exposed to vascular disease risk. NO release can be induced by brachial artery flow increase, induced by forearm cuff inflation and release. The resulting flow mediated brachial lumen diameter change (FMD) can be measut'ed with ultrasound. Brachial diameter and FMD repeatability of the radio fi'equency (RF) based wall track system (WTS, Pie Medical) was calculated with the aim to estimate the power of the method to detect effects of vasoprotective agents in healthy adults. Methods: On two separate occasions (_>1 day between observations in each subject) one sonographer measured FMD in 15 subjects (age 32.1(range 22-59)years, 2 BMI 23.93(SD2.64) kg/m ). The brachial artery was visualised in B-mode (Esaote AU5, 7.5MHz probe). Brachial arterial flow was provo- cated with 5 minute inflation of a forearm blood pressure cuff at 300mmHg. Thlee brachial lumen diameter observations wele done prior cuff inflation; llwere done after cuff release. RF signals of the brachial arterial walls were saved with the WTS for off-line analyses (RF Markview, Vascular Lab Internal Medicine, Groningen University Hospital). FMD was defined as the % change between maximal post-cuff release and the averaged pre-cuff inflation brachial lumen diameters. Results: Brachial lumen diameter was 4.042 (SD0.478) mm on first and 4.077 (0.554) mm on second occasion. The means of the (per subject) pah'ed lepeated measut'ements was 0.034 (0.254) mm. For FMD these numbers were 5.83 (SD3.26) FMD5.21 (3.36) and 0.62(3.90)%, respectively. Conclusions: Assuming c~=0.05, ~=0.2 (power 80%), it was estimated FMD WTS measut'ements in 30 healthy adults are requh'ed to detect a clinically relevant effect size of 2.5% in FMD. •] CARDIOVASCULAR RISK FACTORS (CRF) AFTER KIDNEY-PANCREAS TRANSPLANTATION (KPT) A. Caballero, J. Gonz lez-Posadas, D. Hern ndez, L. P rez-Tamaj n, A. L pez-Alba, J. P rez-Luis, A. Alarc , M. Meneses, L. Morcill. Department of Endocrinology, Nefrology, Surgery" Hospital Universitario Canarias, Tenerife, Espa a Cardiovascular morbility and mortality in type 1 diabetic patient with end- stage renal disease can be reduced after KPT, although mechanism is not well known. We report on 9 type 1 diabetic patients (age:37,3-4- 7 years; males/females: 4/5; BMI:23,9-4- 2,1 kg/m2; dut'ation of diabetes: 24,3-4- 5,8 years) who received KPT (immunosupression: tacrolimus, mycophenolate- mophetil and steroids) and wele studied before transplantation and at 6 months. Before KPT all patients needed antihypertensive therapy and 8 out of 9 needed statins. Total cholesterol (TC), LDLc, HDLc, triglycerides (TG), apoprotein A (ApoA) and apoprotein B (ApoB) were determined with other CRF (systolic blood pressut'e [SBP], diastolic blood pressure [DBP] and body mass index [BMI]). Results are shown in tables: Glucose HbAlc SBP DBP BMI Before TRP 246± 89 8,4± 1,2 156± 7 96± 7 23± 2 6 month 80± 81 4,7± 0,61 137± 15 2 79± 91 25± 3 3 Wilcoxon: (i) p<0,007; (2) p<0,0i; (3) p< 0,05. TC LDLc HDLc TG Before TRP 189± 63 89± 47 63± 16 130± 51 6 month 175± 37 99± 27 59± 12 88± 333 Normalization of fasting plasma glucose and HbAlc (p<0.007) with no exogenous insulin administration was achieved after KPT. Significant improvement of TG, SBP and DBP was also observed and BMI increased. TC, HDLc, LDLc, ApoA and ApoB were similar before and after trans- plantation. Moreover, after grafting, 8 out of 9 patients quit antihypertensive therapy and none need statins. In conclusion, these results suggest KPT can early improve CRF such as HTA and TG and help to achieve an adequate lipid profile (TC, HDLc, LDLc and TG) without no need of statins. • AMBULATORY BLOOD PRESSURE PATTERN IN PATIENTS WITH RESISTANT HYPERTENSION AS A FUNCTION OF THE CIRCADIAN TIME OF ANTIHYPERTENSIVE THERAPY C. Calvo, R. Hermida, D. Ayala, M. Dominguez, M. Covelo, J. Lopez. Hypertension and Vascular Risk Unit, Hospital CI nico Universitario, Santiago, Bioengineering & Chronobiology Labs., Univ. Vigo, Vigo, Spain Patients with resistant hypertension represent an important clinical problem due to their high cardiovascular risk and high prevalence of a non-dipper blood pressut'e (BP) pattern (<10% decline in the noctut'nal relative to the diut'nal BP mean). Recent results indicate that non-dipping in treated hypertensive patients may be lelated to the absence of 24-hour therapeutic coverage [J Hypertens. 2002;20:1097-1104]. Accordingly, we studied the impact of the time of antihypertensive treatment on the cfl'cadian pattern of BP in patients with resistant hypertension. We studied 444 patients with resistant hypertension (228 men), 58.9-4-11.0 years of age. Among them, 210 patients received all theft" medication upon awakening. In the remaining 234 patients, one drug was administered before bedtime. BP was measut'ed at 20- min intervals fi'om 07:00 to 23:00 hours and at 30-min intervals at night for 48 consecutive hour's. Physical activity was simultaneously monitored evely minute by wrist actigraphy, and the information used to determine diurnal and nocturnal means of BP for each patient according to individual resting time. In comparison with patients who received all theft" antihypertensive medication upon awakening, subjects with one drug before bedtime were characterized by a significant reduction in the 24-hour" mean of systolic BP (4.9 mm Hg, P<0.001). This reduction was much more prominent during noctut'nal resting hour's (7.5 mm Hg, P< 0.001). Patients receiving one drug before bedtime also showed a significant reduction in the 24-hour mean of pulse pressut'e (4.3 mm Hg, P<0.001). 82.9% of the patients with all drugs on awakening were non-dippers. This percentage was significantly reduced to 51.7% in patients who leceived one antihypertensive drug before bedtime (P< 0.001). In patients with resistant hypertension, pharmacological therapy should take into account when to treat with respect to the rest-activity cycle of each patient, as a function of the therapeutic coverage of the drugs and the baseline cfl'cadian BP profile of the patient. This chronopharrnacology 74th EAS Congress, 17-20 April 2004, Seville, Spain