Copyright 2016 by ESPGHAN and NASPGHAN. Unauthorized reproduction of this article is prohibited. ESPGHAN 2012 Guidelines for Coeliac Disease Diagnosis: Validation Through a Retrospective Spanish Multicentric Study  Ester Donat, y Jose M. Ramos, z Fe´lixSa´nchez-Valverde, § Ana Moreno, jj Maria-Jose Martinez, ô Rosaura Leis, # LuisPen˜a-Quintana, yy Gemma Castillejo, zz SoniaFerna´ndez, §§ Zurin˜eGarcia, jjjj Luis Ortigosa, ôô Elena Balmaseda, ## Jose´-ManuelMaruga´n,  Francisco-Javier Eizaguirre, yyy Helena Lorenzo, zzz Josefa Barrio, and §§§ Carmen Ribes-Koninckx, on behalf of the SEGHNP Working Group on Coeliac Disease ABSTRACT Objectives: A large retrospective multicentre study was conducted in Spain to evaluate the efficiency of the new European Society for Pediatric Gastroenterology, Hepatology, and Nutrition (ESPGHAN) criteria for the diagnosis of coeliac disease (CD). Methods: The study protocol was approved by the ethics committee of Hospital Universitari i Polite `cnic La Fe (Valencia, Spain). The present study included 2177 children (ages 0.6–15.9 years) with small bowel biopsy (SBB) performed for diagnostic purposes (from 2000 to 2009) and with a minimum 2-year follow-up after biopsy. Results: CD was diagnosed in 2126 patients (97.5%) and excluded in 51 (2.5%). Tissue transglutaminase antibodies (TG2A), anti-endomysial antibodies (EMA), and human leukocyte antigen (HLA) were reported in 751 patients, 640 symptomatic and 111 asymptomatic. TG2A levels >10 times the upper limit of normal, plus positive EMA and HLA DQ2 and/or DQ8 haplotypes, were found in 336 symptomatic patients, all of them with Received January 20, 2015; accepted May 17, 2015. From the  Pediatric Gastroenterology, Hepatology and Nutrition, Hospital Universitari i Polite `cnic La Fe, Valencia, the y Pediatric Gastroenterology, Complejo Hospitalario Universitario de Vigo, Vigo, the z Pediatric Gastroenterology, Hospital Virgen del Camino, Pamplona, the § Pediatric Gastro- enterology and Nutrtion, Hospital Teresa Herrrera, A Corun ˜ a, the jj Pediatric Gastroenterology, Hospital Universitario Nin ˜ o Jesus, Madrid, the ô Pediatric Gastroenterology, Hepatology and Nutrition Unit, Pediatrics Department, Hospital Clı ´nico Universitario de Santiago, Pediatrics Nutrition Research Group- IDIS, Universidad de Santiago de Compostela, Santiago de Compostela, the # Pediatric Gastroenterology and Nutrition Unit, Hospital Materno-Infantil, Universidad de Las Palmas de Gran Canaria, Las Palmas and CIBER OBN, the yy Pediatric Gastroenterology, Hospital Universitari de Sant Joan de Reus, Reus, the zz Pediatric Gastroenterology, Hospital Severo Ochoa, Madrid, the §§ Pediatric Gastroenterology, Hospital de Txagorritxu, Vitoria, the jjjj Pediatric Gastroenterology, Hospital Universitario Ntra Sra de Candelaria, Tenerife, the ôô Pediatric Gastroenterology, Hospital General de Albacete, Albacete, the ## Pediatric Gastroenterology, Hospital Clı ´nico Universitario de Valladolid, Valladolid, the  Pediatric Gastroenterology Unit, Hospital Donostia, San Sebastian, the yyy Pediatric Gastroenterology, Hospital Universitario de Basurto, Bilbao, the zzz Pediatric Gastroenterology, Hospital Universitario de Fuenlabrada, Madrid, and the §§§ Pediatric Gastroenterology, Hepatology and Nutrition, Hospital Universitari i Polite `cnic La Fe, Valencia, Spain. Address correspondence and reprint requests to Ester Donat, Pediatric Gastroenterology, Hepatology and Nutrition, Hospital Universitari i Polite `cnic La Fe, Bulevar Sur, Valencia 46026, Spain (e-mail: donat_est@gva.es). Supplemental digital content is available for this article. Direct URL citations appear in the printed text, and links to the digital files are provided in the HTML text of this article on the journal’s Web site (www.jpgn.org). The following authors are listed as collaborating authors from the Spanish Paediatric Gastroenterology, Hepatology, and Nutrition Society Working Group on Coeliac Disease: Cesar Sa ´nchez (Hospital Gregorio Maran ˜on, Madrid); Oscar Manrique (Hospital General Universitario de Alicante); Mo ´nica Garcı ´a Peris (Hospital Lluis Alcanyis de Ja ´tiva, Valencia); Mercedes Juste (Hospital San Juan de Alicante); Cesar Perez (Hospital Virgen de los Lirios de Alcoy, Alicante); Eduardo Ubalde (Hospital San Jorge, Huesca); M A ´ ngeles Go ´mez (Hospital de Yecla, Murcia); Isabel Polanco (Hospital Infantil Universitario La Paz, Madrid); Carmen Jovanı ´ (Hospital de la Plana de Villareal, Castello ´n); Miguel Calabuig (Hospital Universitario General, Valencia); Jose Carlos Salazar (Hospital Infantil Virgen del Rocio, Sevilla); Javier Torrecilla (Hospital Virgen de la Luz, Cuenca); Miriam Blanco Rodrı ´guez (Fundacio ´ n Jime ´nez Dı ´az, Madrid); Antonio Milla ´n (Hospital Universitario de Valme, Sevilla); Pablo Ferrer (Hospital General de Requena, Valencia); Soraya Cuadrado (Hospital Nuestra Sen ˜ora del Prado, Talavera de la Reina, Toledo); Miguel A ´ ngel Lo ´pez (Hospital Virgen de las Nieves, Granada); Jose I. Garcia-Burriel (Hospital Universitario de Vigo); Alfonso Solar (Hospital Teresa Herrrera, Corun ˜a); Rosa Ana Mun ˜oz-Codoceo (Hospital Nin ˜o Jesu ´s, Madrid); Lydia Monasterio (Hospital Clı ´nico Universitario de Santiago); Miguel Bolonio and Etna Masip (Hospital Universitari i Polite `cnic La Fe, Valencia); Sonia Marı ´a Rivero (Hospital Materno Infantil de Las Palmas de Gran Canaria); Ana Isabel Rayo (Hospital Severo Ochoa, Madrid); Idoia Huald (Hospital de Txagorritxu, Vitoria); Jose ´ Ramo ´n Alberto (Hospital Ntra Sra de Candelaria); Inmaculada Navarro (Hospital General de Albacete); Carmen Calvo (Hospital Clı ´nico Universitario de Valladolid); Nuria Chaves (Hospital de Donostia); Izaskun Anguiano (Hospital Universitario de Basurto, Bilbao); Myriam Herrero (Hospital Universitario de Fuenlabrada, Madrid); Mar Tolı ´n (Hospital Gregorio Maran ˜on); Fernando Clemente and Ester Pe ´rez (Hospital General Universitario de Alicante); Marcelina Algar (Hospital Infantil Universitario La Paz, Madrid); Rosa Segarra (Hospital de la Plana de Villareal, Castello ´n); Alejandro Rodriguez and Beatriz Espin Jaime (Hospital Infantil Virgen del Rocio, Sevilla); M Jose Manzano (Hospital Universitario de Valme, Sevilla); Julio Romero, Jose Maldonado Lozano, Nazareth Martino ´n Torres, and Pilar Pavon (Hospital Clı ´nico Universitario de Santiago). The authors report no conflicts of interest. Copyright # 2016 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition DOI: 10.1097/MPG.0000000000000870 ORIGINAL ARTICLE:GASTROENTEROLOGY 284 JPGN  Volume 62, Number 2, February 2016