Int J Cur Res Rev | Vol 13 • Issue 02 • January 2021 90 Lipid Peroxidation and Hepatoprotective Activity of Bauhinia Vahli Against Carbon Tetra Chloride Induced Toxicity Amit Kumar Nigam 1,2 , Phool Chandra 1 , Zeashan Hussain 3 , Neetu Sachan 1 1 School of Pharmaceutical Sciences, IFTM University, Lodhipur Rajput, Delhi Road (NH-24), Moradabad (UP)-244192, India; 2 Goel Institute of Pharmacy & Sciences, Faizabad Road, Near Indira Canal, Lucknow, Uttar Pradesh, India; 3 Mahatma Gandhi Institute of Pharmacy, Lucknow, Uttar Pradesh, India. Corresponding Author: Amit Kumar Nigam, School of Pharmaceutical Sciences, IFTM University, Lodhipur Rajput, Delhi Road (NH-24), Moradabad, Uttar Pradesh, India; Phone: +91-7905148344; Email: amitnigam2511@gmail.com ISSN: 2231-2196 (Print) ISSN: 0975-5241 (Online) Received: 22.07.2020 Revised: 19.09.2020 Accepted: 20.11.2020 Published: 16.01.2021 INTRODUCTION The liver is the largest organ in the body weighing 1400- 1600 gm. in males and 1200-1400 gm. in females. There are two main anatomical lobes right lobe and left lobe, the right is being about six times the size of the left lobe. It produces bile, an alkaline compound which aids in digestion, via the emulsification of lipids. Besides, it aids the metabolism of carbohydrate, protein and fat, detoxification, secretion of bile, and storage of vitamins. 1,2 The primary function of this organ in the removal of substances from the portal circula- tion makes it susceptible to first and persistent attacks by offending foreign compounds, culminating in liver dysfunc- tion. 3 Liver cirrhosis has become a serious health problem because of the wider use of prescribed medications with adverse reac- tions in the modern life of today or drug misuse. The current research has targeted on finding new therapeutic alternatives and analyzing their mechanism to get rid of the signalling routes and reduce the loss induced on the liver. 4 The num- bers of compounds of natural origin are generally used as possible health care options and they are being experimented on various animal models. 5,6 Many medicinal plants with hepatoprotective activity have been reported by several re- searchers. 7,8 In continuation of the search of hepatoprotec- tive agents, Malu Creeper was undertaken to investigate the active constituents and its medicinal value. Bauhunia vahlii (Malu Creeper) is a tree of large family Leguminosaceae Original Research International Journal of Current Research and Review DOI: http://dx.doi.org/10.31782/IJCRR.2021.13201 IJCRR Section: Healthcare ISI Impact Factor (2019-20): 1.628 IC Value (2019): 90.81 SJIF (2020) = 7.893 Copyright@IJCRR ABSTRACT Introduction: Liver cirrhosis has become a serious health problem because of the wider use of prescribed medications with adverse reactions in the modern life of today or drug misuse. Objective: In the present study, the hepatoprotective activity of the aerial part of Bauhinia vahlii was investigated against the carbon tetrachloride-induced hepatotoxicity model in Wistar albino mice. Methods: Glutathione (GSH), Glutathione Peroxidase (GPx), Glutathione S-transferase (GST), Superoxide dismutase (SOD), Multiple Organ Dysfunction Syndrom (MOD) were measured in liver and blood samples. The acute toxicity study of the selected plant showed that there is no mortality or adverse reaction at the fixed dose of 2000 mg/kg. The methanolic extract at 200 and 400 mg/kg body weight respectively were administered daily to study the hepatoprotective effect in carbon tetrachloride-induced hepatotoxic model for 7 days. Results: A remarkable decrease was observed in bilirubin, alkaline phosphatase (ALP), serum glutamic-oxaloacetic transami- nase (SGOT), and serum glutamic-pyruvic transaminase (SGPT) levels in the treatment group as compared to the hepatotoxic group. In a histopathological study, hepatocytic necrosis and inflammation in the centrilobular region with portal triaditis was found in hepatotoxicity induced group whereas minimal inflammation with moderate portal triaditis and normal lobular architec- ture observed in extract treated groups. Conclusion: From the observation, it can be concluded that the methanolic extract of Bauhinia vahlii has the potential to revert the hepatic injury induced by carbon tetrachloride. Comparatively, the methanolic extract at 400 mg/kg w/w resulted in a signifi- cant hepatoprotective effect. The standard drug silymarin was used for comparison. Key Words: Hepatoprotective activity, Lipid peroxidation, Bauhinia vahlii, Silymarin, Carbon tetrachloride