ABR Vol 6 [1] January 2015 15 | P age ©2015 Society of Education, India Advances in Bioresearch Adv. Biores., Vol 6 (1) January 2015: 15-18 ©2015 Society of Education, India Print ISSN 0976-4585; Online ISSN 2277-1573 Journal’s URL:http://www.soeagra.com/abr.html CODEN: ABRDC3 ICV 7.20 [Poland] ORIGINAL ARTICLE Evaluation of Anti Asthmatic Effect of Extra Virgin Olive Oil (Olea europea) Against Milk Induced Leukocytosis and Eosinophilia Farooq Ahmed Wani 1 , Shaik Rahiman 2 , Bilal Ahmad Tantry 3 1 Department of Pathology, College of Medicine, Aljouf University, Saudi Arabia 2 Department of Biochemistry, College of Medicine, Aljouf University, Saudi Arabia 3 Department of Microbiology, College of Medicine, Aljouf University, Saudi Arabia Email: fawani@ju.edu.sa ABSTRACT From the last few decades, the management of chronic pain has remained a challenge for medical science research. Currently, available analgesic drugs such as NSAIDs have few adverse effects. As a result, the traditional system has gained importance in the field of medicine. To evaluate the anti asthmatic activity of extra virgin olive oil (Olea Europea) for the management of bronchial asthma. In the current study, commercially available virgin olive oil (1.3 ml/kg p.o) was screened for the management of bronchial asthma using milk induced leukocytosis and eosinophilia in mice. Our study results have shown that test olive oil dose (1.3 ml/kg p.o) significantly decreased milk induced leukocytosis and eosinophilia in mice when compared with control group. Conclusions: We conclude that olive oil has anti asthmatic activity against milk induced leucocytosis and eosinophilia. further, working is going on various asthmatic models to evaluate its efficacy in the management of asthma. Keywords: Asthma, Extra Virgin Olive oil, Leucocytosis, Eosinophilia. Inflammation. Received 21/11/2014 Accepted 21/12/2014 ©2014 Society of Education, India How to cite this article: Farooq Ahmed W, Shaik R, Bilal Ahmad T. Evaluation of Anti Asthmatic Effect of Extra Virgin Olive Oil (Olea europea) Against Milk Induced Leukocytosis and Eosinophilia. Adv. Biores., Vol 6 [1] January 2015: 15-18. DOI: 10.15515/abr.0976-4585.6.1.1518 INTRODUCTION Bronchial asthma is characterized by airway hyper-responsiveness leading to inflammation and narrowing of airways [1]. Asthma affects about 300 million people worldwide and it has been estimated that a further 100 million will be affected by 2025 [2]. Environmental and genetic factors together play an important role in the etiology of asthma [3]. Nowadays various types of anti asthmatic drugs are available in the market for the management of asthma. However, all of these are limited to short symptomatic relief and result in complex side effects. The adverse effects of these synthetic drugs prompt a switch over to traditional complementary and alternative medicine [4]. In spite of voluminous literature on the subject, the treatment of asthma continues to be far from satisfactory. Therefore, there is a dire need to identify effective and safe remedies to treat bronchial asthma [4]. In Mediterranean countries Olive oil has been widely used in traditional medicine in the management of several diseases [5]. Olive oil has been commercialized as a food supplement which can be consumed in the form of syrup and capsules. Previous studies reported the potential activities of olive oil (Olea europea) such as antioxidant [6], hypotensive [7], cardiovascular [8] , hepato-protective [9], hypoglycemic activity [10] anti inflammatory [11] and anti microbial activities [12]. Several studies have shown that people who consumed about 2 tablespoons of virgin olive oil daily for 1 week showed less oxidation of LDL cholesterol [13]. Olive oil is very well tolerated by the stomach. In fact, olive oil has a protective function and beneficial role in gastric ulcers and gastritis [14]. Only few studies reported that ripe olives have anti histaminic activity [15]. More studies are needed to explore the exact role of olive oil in asthma. Keeping in view, the present study was aimed to evaluate the anti-asthmatic activity of extra virgin olive oil using milk induced leukocytosis and eosinophilia in mice. Advances in Bioresearch