Borneo Journal of Pharmacy https://journal.umpr.ac.id/index.php/bjop/article/view/3499 Vol 6 Issue 1 February 2023 DOI: https://doi.org/10.33084/bjop.v6i1.3499 Pages 8 – 14 e-ISSN: 2621-4814 How to cite: Susanty A, Febrina M, Putri DS, Ikhtiarudin I, Wahyuni FS, Dachriyanus. Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells. Borneo J Pharm. 2023;6(1):8-14. doi:10.33084/bjop.v6i1.3499 INTRODUCTION Breast cancer attacks the epithelial tissue of the breast (carcinoma) and generally originates from the glands, gland ducts, and supporting tissues of the breast 1 . The risk factors for breast cancer include a family history of breast cancer, obesity, consumption of fast food that contains a lot of saturated fat, alcohol consumption, menopause at an older age (> 50 years), early menarche, namely the first menstruati on at a relatively young age (≤12 years), long-term use of hormonal contraceptives, radiation exposure, having had a benign breast tumor or breast cancer, never giving birth or giving birth for the first time at the age of more than 35 years, and not breastfeeding are also risk factors for breast cancer 2,3 . Treatment of cancer patients can be done with surgery, radiotherapy, and chemotherapy. Chemotherapy is a cancer treatment using cytotoxic chemicals 4 . The working principle of chemotherapy is to kill cancer cells, control their growth, and stop their growth from spreading or reduce the symptoms caused by cancer 5 . Treatment with chemotherapy has not given satisfactory results because it does not work precisely. It can also cause normal cell damage and cause some side effects, such as hair loss, nausea, vomiting, diarrhea, susceptibility to infection, thrombocytopenia, neuropathy, and myalgia. Treatment with radiation causes side effects such as nausea and vomiting 6 . Meanwhile, surgical treatment cannot entirely remove body tissue damaged by cancer 7 . Because of these conditions, it is necessary to look for alternative drugs, one of which is developing anticancer agents derived from natural ingredients or Cytotoxic Activity of Ethyl Acetate Extract from Voacanga foetida (Bl.) Rolfe Leaves Against T47D Breast Cancer Cells Adriani Susanty 1* Mira Febrina 1 Dian Sanita Putri 1 Ihsan Ikhtiarudin 1 Fatma Sri Wahyuni 2 Dachriyanus 2 1 Department of Pharmacy, Sekolah Tinggi Ilmu Farmasi Riau, Pekanbaru, Riau, Indonesia 2 Department of Pharmacy, Universitas Andalas, Padang, West Sumatra, Indonesia * email: adrianisusanty@stifar-riau.ac.id Keywords: Breast cancer Cytotoxic MTT Assay Voacanga foetida Tampa Badak Abstract The cytotoxicity of ethyl acetate extract from Tampa badak (Voacanga foetida (Bl.) Rolfe) leaves against Artemia salina leach larvae was determined using the brine shrimp lethality test (BSLT) method and was evaluated against T47D breast cancer cells using MTT assay method. The result of BSLT showed a consistent result with MTT assay, which is the result obtained that ethyl acetate extract is very toxic against A. salina Leach larvae with LC50 value of 8.61 µg/mL and very cytotoxic against T47D breast cancer cells with IC50 values of 0.87; 0.66; and 0.95 µg/mL at the 24, 48 and 72 hours of incubation times, respectively. The MTT assay data were analyzed using a two- way ANOVA statistical method to see the effect of the dependent variable (concentration and time) on the independent variable (% viability). Based on the statistical test result, there is a difference in % viability between concentrations of 0.1; 1; and 10 µg/mL (p <0.05), but the length of incubation does not affect % viability (p >0.05). Received: April 27 th , 2022 1 st Revised: November 22 nd , 2022 Accepted: January 14 th , 2023 Published: February 28 th , 2023 © 2023 Adriani Susanty, Mira Febrina, Dian Sanita Putri, Ihsan Ikhtiarudin, Fatma Sri Wahyuni, Dachriyanus. Published by Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya. This is an Open Access article under the CC-BY-SA License (http://creativecommons.org/licenses/by-sa/4.0/). DOI: https://doi.org/10.33084/bjop.v6i1.3499 Research Article