Jurnal Ilmiah Farmasi (Scientific Journal of Pharmacy) 18(2) Agustus-Desember 2022, 219-233 ISSN: 1693-8666 available at http://journal.uii.ac.id/index.php/JIF 219 Characterization and determination of total flavonoid content of extract and fraction of papaya jantan flower (Carica papaya L.) using UV-Vis spectrophotometry Karakterisasi dan penetapan kadar flavonoid total ekstrak dan fraksi bunga pepaya jantan (Carica papaya L.) dengan spektrofotometri UV-Vis Dilla Nur Pratiwi 1 , Nastiti Utami 1 *, Diah Pratimasari 1 1 Program Studi S1 Farmasi, Sekolah Tinggi Ilmu Kesehatan Nasional, Surakarta, Jawa Tengah *Corresponding author: nastiti.utami@stikesnas.ac.id Abstract Background: The potential of pepaya jantan flowers (Carica papaya L.) as functional herbal ingredients, such as antioxidants, is influenced by the activity of secondary metabolites, one of which is flavonoids. Objective: This study aims to determine the total flavonoid content and characterization of flavonoids in the ethanol extract and flower fraction of papaya jantan (Carica papaya L.). Method: Pepaya jantan flower was extracted by maceration using ethanol 70% then fractionated using multilevel fractionation. The identification of flavonoids was tested using Taubeck, Wilstater, and TLC. Extract and fractions were determined of flavonoid content using the colorimetric method and identification of flavonoids based on the wavelength of cinnamoyl and benzoyl in the spectrum UV-Vis. Results: The results of the determination of flavonoid content showed that ethanol extract was the highest at 0.6805 ± 0.0045 %QE and n-hexane fractions were the lowest at 0.4178 ± 0.0058 %QE. Identification of flavonoids using the UV-Vis spectroscopy method showed the absorption of substituted 3-OH flavonol and free 3-OH flavonol. Conclusion: The highest flavonoid content was found in the ethanol extract of papaya jantan flower. The types of flavonoids in the extract and fractions of male papaya flower indicated the type of flavonol based on the wavelength of cinnamoyl and benzoyl. Keywords: Carica, flavonoid, cinnamoyl, benzoyl, spectrophotometry UV-Vis Intisari Latar belakang: Potensi bunga pepaya jantan (Carica papaya L.) sebagai antioksidan dipengaruhi metabolit sekunder, salah satunya flavonoid. Tujuan: Penelitian ini bertujuan untuk mengetahui kadar flavonoid total serta karakterisasi flavonoid dalam ekstrak etanol dan fraksi bunga pepaya jantan (Carica papaya L.). Metode: Bunga pepaya jantan dilakukan maserasi dengan etanol 70%, dilanjutkan fraksinasi bertingkat. Identifikasi flavonoid dilakukan menggunakan metode Taubeck, Wilstater dan KLT. Kadar flavonoid ekstrak dan fraksi ditetapkan menggunakan metode kolorimetri serta identifikasi flavonoid berdasarkan pita cinnamoyl dan benzoyl pada spektrum UV-Vis. Hasil: Hasil penetapan kadar flavonoid menunjukkan bahwa ekstrak etanol memiliki kadar tertinggi sebesar 0,6805 ± 0,0045 %QE, serta kadar terendah dalam fraksi n-heksan sebesar 0,4178 ± 0,0058 %QE. Identifikasi flavonoid metode spektrofotometri UV-Vis menunjukkan penyerapan flavonol 3-OH substitusi dan flavonol 3- OH bebas. Kesimpulan: Kadar flavonoid tertinggi ditemukan dalam ekstrak etanol bunga pepaya. Jenis flavonoid dalam ekstrak dan fraksi bunga pepaya jantan mengindikasikan jenis flavonol berdasarkan panjang gelombang dari cinnamoyl dan benzoyl. Kata kunci : carica, flavonoid, cinnamoyl, benzoyl, Spektrofotometri UV-Vis 1. Pendahuluan Pengembangan obat tradisional harus memenuhi syarat yaitu aman, berkhasiat dan bermutu, sehingga pengembangan obat tradisional perlu didukung adanya bukti ilmiah untuk mengetahui