Vet Integr Sci Vet Integr Sci Win et al. Vet Integr Sci. 2023; 21(2): 439 - 456 Veterinary Integrative Sciences 439 Veterinary Integrative Sciences 2023; 21(2): 439 - 456 DOI; 10.12982/VIS.2023.031 Vet Integr Sci Veterinary Integrative Sciences ISSN; 2629-9968 (online) Website; www.vet.cmu.ac.th/cmvj Research article Prevalence and risk factors of brucellosis and dairy farmers’ KAP in 2 townships, Myanmar Hlaing Win 1,2 , Suwicha Kasemsuwan 2 , Hnin Thidar Myint 1 , Monaya Ekgatat 3 and Waraphon Phimpraphai 2,* 1 Livestock Breeding and Veterinary Department, Naypyitaw 15015, Myanmar 2 Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand 3 National Institute of Animal Health, Department of Livestock Development, Bangkok 10220, Thailand Abstract Brucellosis is a highly infectious zoonotic disease and has a great economic impact on dairy production. In Yangon Region of Myanmar, 0.47% seroprevalence of brucellosis in dairy cattle and 8 human IgG positive cases was reported. This study aims to determine the prevalence of brucellosis in dairy farms and its risk factors as well as to assess the current knowledge, attitudes and practices (KAP) of dairy farmers regarding brucellosis. Our study was conducted in Hlegu and Taikkyi Township during September 2019 to January 2020. Pooled milk samples were collected from 174 dairy farms. Using Milk Ring Test (MRT), Brucella spp. was found in 30 dairy farms and the prevalence was 17.2%. Statistically significant farm factors associated with MRT positive results were farm location in Takkyi Township (Odds ratio (OR) 3.19; 95%CI = 1.42, 7.15), pasture sharing (OR 3.43; 95%CI = 1.34, 8.76), and abortion (OR 2.68; 95% CI = 1.21, 5.98). Survey for the KAP for brucellosis in farmers indicated that good knowledge, attitudes and practices were in 35.1, 99.4, and 62.1% of the respondents, respectively. In conclusion, brucellosis was still a problem of dairy farms in study area and MRT can be used as a farm level screening test. Educational campaign for brucellosis prevention and control should be considered according to a lack of knowledge and inappropriate farm practices among dairy farmers Keywords: Brucellosis, KAP, Milk Ring Test, Myanmar, Risk factors Open Access Copyright: ©2023 Author (s). This is an open access article distributed under the term of the Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author (s) and the source. Corresponding author: Waraphon Phimpraphai, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand. E-mail: fvetwrp@ku.ac.th. Article history; received manuscript: 8 March 2022, revised manuscript: 18 July 2022, accepted manuscript: 3 March 2023, published online: 16 March 2023 Academic editor; Korakot Nganvongpanit