p-ISSN 1693-5683; e-ISSN 2527-7146 78 Vol. 20, No. 1, May 2023, pp. 78-83 Research Article Wound Healing Effectivity of Ageratum conyzoides L. Leaf Ethanolic Extract (Purple Flower Type), Centella asiatica (L.) Urban Leaf Ethanolic Extract, and Astaxanthin Combination Gel Preparation in Diabetic Animal Model Sarah Sahila 1 , Hendy Suhendy 2 , Yedy Purwandi Sukmawan 1* 1 Department of Pharmacology and Clinical Pharmacy, Bakti Tunas Husada Health Science College, Cilolohan Street No. 36, Tasikmalaya, 46184, West Java, Indonesia. 2 Department of Pharmacognosy, Bakti Tunas Husada Health Science College, Cilolohan Street No. 36, Tasikmalaya, 46184, West Java, Indonesia. https://doi.org/10.24071/jpsc.003319 J. Pharm. Sci. Community, 2023, 20(1), 78-83 Article Info ABSTRACT Received: 23-04-2021 Revised: 08-06-2021 Accepted: 22-06-2021 *Corresponding author: Yedy Purwandi Sukmawan email: yedipur@gmail.com Keywords: Ageratum conyzoides; astaxanthin; Centella asiatica; diabetic; wound healing The diabetic wounds are one of the global burden diseases. In our previous study, Ageratum conyzoides L. leaf ethanolic extract (purple flower type) 10%, Centella asiatica (L.) Urban leaf ethanolic extract 5%, and astaxanthin 0.1% combination gel preparation showed remarkable wound healing effectivity. However, this wound healing effectivity in the diabetic condition is still unknown. There were three groups used, namely negative group (placebo/gel base), positive group (oxoferin/tetrachlorodecaoxide), and test group (Ageratum conyzoides L. leaf ethanolic extract (purple flower type) 10%, Centella asiatica (L.) Urban leaf ethanolic extract 5%, and astaxanthin 0.1%). Each group consisted of three male Wistar rats. We conducted diabetes induction with alloxan (175 mg/Kg BW i.p) and followed by an incision 1.5 cm on the back. All of these groups were given treatment once daily and monitored for 14 days. The results of the study showed the test group indicated significantly better effectivity than positive and negative groups (p<0.05). The percentages of the wound healing effectivity of the test, positive and negative groups were 62.74%, 51.77%, and 41.65%, respectively. Ageratum conyzoides L. leaf ethanolic extract (purple flower type) 10%, Centella asiatica (L.) Urban leaf ethanolic extract 5%, and astaxanthin 0.1% combination gel preparation has excellent potential to be developed as a commercial product to treat diabetic wound conditions. INTRODUCTION Diabetes is one of the top 10 leading causes of death worldwide (Forouzanfar et al., 2016). Global incidence, prevalence, death, and disability-adjusted life-years were projected to increase in 2025 to 26.6 million, 570.9 million, 1.59 million, and 79.3 million in 2025, respectively (Lin et al., 2020). Diabetic wounds or diabetic foot ulcers are a common complication in diabetic patients with the prevalence of this condition reaching 11.6% and the prevalence increased over time (Abdissa et al., 2020; Lin et al., 2020). It is estimated that the prevalence of this condition in Asia will reach over 5.5% (95%CI: 4.6-6.4%) (Zhang et al., 2017). This condition is related to an increase in amputation incidence and mortality (Robbins et al., 2008; Kim et al., 2018). In a cross-sectional survey study conducted by Bondor et al. (2016), that involved 21,174 patients, 14.85% reported a history of foot ulcers and 3.6% reported an amputation. Moreover, the diabetic foot ulcer relates to an increase of 5-year mortality rate between 43-55% (Robbins et al., 2008).