1 Specific anion effect on properties of HRV 3C protease Eva Dušeková 1, ⁋ , Martin Berta 1, ⁋ , Dagmar Sedláková 2, ⁋ , David Řeha 3 , Veronika Dzurillová 1 , Anastasiia Shaposhnikova 3,4 , Fatemeh Fadaei 3,4 , Mária Tomková 5 , Babak Minofar 3* , Erik Sedlák 5* 1 Department of Biophysics, Faculty of Science, P. J. Šafárik University in Košice, Jesenná 5, 04154 Košice, Slovakia 2 Department of Biophysics, Institute of Experimental Physics, Slovak Academy of Sciences, Watsonova 47, 040 01 Košice, Slovakia 3 Faculty of Science, University of South Bohemia in České Budějovice, Branišovská 1645/31A, 37005 České Budějovice, Czech Republic. 4 Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology of the Czech Academy of Sciences, Zámek 136, 37333 Nové Hrady, Czech Republic 5 Centre for Interdisciplinary Biosciences, P. J. Šafárik University in Košice, Jesenná 5, 04154 Košice, Slovakia ⁋ The authors contributed equally to this work * To whom correspondence should be addressed: Faculty of Science, University of South Bohemia, Branišovská 1760, 37005 České Budějovice, Czech Republic; email: babakminoofar@gmail.com Centre for Interdisciplinary Biosciences, P. J. Šafárik University in Košice, Jesenná 5, 04154 Košice, Slovakia; email: erik.sedlak@upjs.sk