Acta Hortic. 1277. ISHS 2020. DOI 10.17660/ActaHortic.2020.1277.46 Proc. XII International Rubus and Ribes Symposium: Innovative Rubus and Ribes Production for High Quality Berries in Changing Environments Eds.: C. Carlen et al. 321 Effect of genotype and environment on the chemical composition of raspberry fruits F. Will 1 , E. Krü ger 1 , K. Kumar 1 , C. Patz 1 and A. Sønsteby 2 1 Hochschule Geisenheim University (HGU), Germany; 2 Norwegian Institute of Bioeconomy Research (NIBIO), Norway. Abstract Four raspberry cultivars were grown at two different latitudes namely in Geisenheim (DE, 49°60’N; 7°57’E) and in Kapp (NO, 60°42’N; 10°52’E) to investigate the impact of these growing sites on primary and secondary fruit chemical ingredients in the 2017 season. Fruits were harvested at two picking dates each with three field replications. Contents of °Brix, glucose, fructose, sucrose, organic acids, ascorbic acid, polyols, total polyphenols, and anthocyanins were analyzed in the fruits. The geographic growing sites, which in this case is more than10 latitudes between HGU in Germany and NIBIO in Norway, has partly no, partly significant effects on the primary and secondary ingredients of the investigated raspberry cultivars. In respect to the created data set, temperatures shortly before or at the picking dates were not considered. It may be expected that temperatures at harvest have an effect on the fruit ingredients and therefore on a further classification of the samples. Keywords: raspberry, genotype, environmental interaction, cultivar, latitude, chemical compound, anthocyanin INTRODUCTION The present study is part of the EU-GoodBerry project “Improving the stability of high- quality traits of berry in different environments and cultivation systems for the benefit of European farmers and consumers” and considers, among others, the meaning of genotype and environment on raspberry quality. It is well-known that genotype affects the chemical composition of fruits. However, until now, for Rubus spp., little is known about the variability of raspberry constituents as affected by climatic factors (Hykkerud et al., 2018) and especially by growing sites at different latitudes. Main effects that change with latitude are temperature and day length including light quality (global radiation, UV -light) and quantity of solar radiation (Mazur et al., 2014). Earlier studies showed that high temperature during fruit development increases the content of secondary plant compounds in raspberry (Remberg et al., 2010). Within GoodBerry, four raspberry cultivars are grown from four partners located at different latitudes: NIBIO, Norway, JHI, Scotland, INHORT, Poland and HGU, Germany. The objective is to investigate the genotype×environment interactions on fruit quality and thus the cultivar consistency. Beside standard quality parameters like Brix, individual sugars and acidity, also ascorbic acid, organic acids, polyols (e.g., myo-inositol, glycerol, erythritol, xylitol, and mannitol), total polyphenols and antioxidant capacity were investigated. Among secondary fruit compounds the focus was laid on anthocyanins, because these typical plant pigments show positive physiological and nutritional purposes. They are also responsible for the visual and sensorial impression of raspberries thus underlining their marketing relevance. In this manuscript, we focus first year’s data, 2017, for the sites HGU in Germany and NIBIO in Norway. MATERIALS AND METHODS Raspberries (cultivars ‘Glen Ample’, ‘Tulameen’, ‘Schö nemann’, and ‘Veten’) were grown in Geisenheim (Germany, 49°60’N; 7°57’E) and further North in Kapp (Norway, 60°42’N; 10°52’E) in 3 experimental plots consisting of 8 canes each with three field replications.