Posters / European Journal of Pharmaceutical Sciences 2 (1994) 117-194 127 I:'33DOES THE~ OFCONDENSABLE FLAVANES CORRELATE WITHTHE CONTENT OF FLAVONOIDES IN CRATAEGI FOUUM CUMFLORE AND THECON'~NT OFPROCYANIDINES IN CRATAEGI FRUCTUS? C. Schilling, M. Tegbneier, G. Harnisdnfeger Depadmentof Pharmaeeutical Technology and Biopharrnacy, Sd'iaper& Br0mmerGrnbH& Co. KG, 38259 Salzgitter, Germany P34THEINFLUENCE OFDIFFERENT EXIliC:nON-PROCEEDINGS ONTHEAMOUNT OFTHUJONE IN EXTRACTS OFABSINTHII HERBA AND THUJAE HERBA M. Teglmeier, G. Harnischfeger Departmentof Pharmaceutical Technology and Biopharmacy, Schaper & Br0mmerGmbH & Co. KG, 38259 Salzgitter, Germany Pharmaceutical preparations of Crataegus are used in the therapy of heartdiseases, when the application of D/gitalis-glykos/des is not yet necessary. Espedally the light forms of stenocardia, coronary-insufficiency or disturbances of heart-functions are primary indications [1]. The d~g Crataegifoliura cura./lore is monographed in the DAB 10, the quality of the Crataegi fruclus is described in the DAC. Flavones, flavonolglycoskles, pmoyanidines, ardnes, catechines, phenolcarbonin acids, ~terpenes and xanthines are known as plant-constituents. The aim of this study was to determine, if the content of condensable flavanes correlates either with the total content of flavonoids, measured according to the DAB 10 for Crataegi folium cura tore, or with the content of pmeyanidines (DAC- monograph for Crotaegifructus). Different batches of the above drugs, whose quality was in accordance with the official monographs, were mixed for the investigations. The samples were pulverized and the total content of the various flavane com/xments were determined according to the methods of the DAB 10 and the DAC: extraction, photomeu'ic quantification and calculation as hyperosid for Crataegi folium cura tore [2] or as cyanidinhydrochlorid for Crataegifructus [3]. The condensable flavanes were measured using a patented gravimetric method. The investigations showed that in principal the contents for these flavanes types correlate. For Crataegi foliura cura flore the mean value of the correlation-coefficient (quotient of the content of condensable flavanes and the total content of the flavonoids for the single sample) amounts to 9.30 with a standard deviation of 1.49. If the correlation (in this case: content of the condensable flavanes divided through the content of procyanidines) is calculated for Crataegifructus, a mean value of 1.67 and a standard deviation of 0.43 were found. Ill Czygan FC, Zeitschrifl fin"Phytotherapie 15, 117-122 (1994) [2] DAB 10, Monographie "Weilklomblitter mit Blfiten" (1991) 13] DAC, Monographie "We'dklornfrfichte" (1991) The con'unon mark of both well known medical herbs is the constituent thujone. Its toxical properties led to the prohibition of absinth-spirits in most countries. 1.25 mg thujonc per kg body weight is the legal limit for the oral application to humans and the LDso-value for the mouse amounts to 87.S mg/kg b.wt. [1,2]. These investigations should show, if the usual oeconomic and industrial proceedings of extraction enrich or reduce thujone in the extracts. The quality of Absinthii herba was in accordance with the specification of the German Pharmacopoeia DAB 10. ~ujae herba, which was harvested from a controlled culfvat/on, was examined by known pharmacognostic and chromatographic methods [3 ]. After cutting the drugs exwacts were prepared by a digestion with ethanol 30% (V/V) at a temperature of 80" C or by percolation with purified water, ethanol 30% (V/V) or ethanol 90% (V/V). Finding the absolute content of thujone in each drug the essential oil was steam-destilled according to the general pharmacopoial method V.4.5.8. For quantifing thujone the exwacts were filtered and samples of the essential oil were diluted with toluene in a ratio of 1 to 10 before measuring by capillary gas-chromatography with FID in accordance to [4]. The steam destination gave the highest contents of thujone 0.24 + 0.09 mg thujone per gram drug for Absinthii herba and 7.59 4- 0.88 mg thujone per gram drug for Thujae herba, whereas the essential oil contained 4.8 % (m/V) in .4bsinthii herba and 20.2 % (m/V) in Thujae herba. If the drags were percolated with purified water or ethanol 30% (V/V) the lowest contents of thujone were measured in both preparations. In the extracts of Absinthii herba no thujone could be detected. The percolates of Thujae herba contained only 0.63 mg + 0.11 thujone per gram drug (purified water as solvent) and 2.79 mg + 0.25 mg thujone per gram drug (ethanol 30% (V/V)). The usual industrial extraction-proceeding, i.e. percolation with ethanol 30% (V/V), reduces the amount of thujonv to 30% in comparison to the content of thujone in the drug. In addition an advantage of the extraction with ethanol 30% (V/V) is a good microbial quality compared with purified water as solvent. 1]Hall RL, 2.Nad. Acad. Sci. (Wash.) 458-459 (1973) [2]Pinto-Scognamiglio W, Boll. Chim. Farm. 106, 292-300 (1967) [3]Baumann Iet al., DAZ 127, 2518-2522 (1987) [4]Tegtmeier M, Hamischfeger G, Pharmanie 49, 56-58 (1994) P35 FLAVONOIDS ANDSOMECHEMICAL ELEMENTS IN INFLORESCEN'nA HELICHRYSI K. Wierzchowska-Renke, I. Kesinski Depaflrnent of Pharmaceutica~ Botany,IdedicaiAcademyof C,-damk, 80-416 C-,dansk, Poland Changes of the contents of flavonoids and biologically active ele- ments such as: Ca, Mg,K, Cu, Fe, Mn and Zn are presented in the paper. The observation of the contents changes have been performed for four years. The inflorescences ofHeliehrysum arenarium were collected from eight natural station located at "Rownina Charzykowska". The investigation were carried separately for red and yellow covers of the capitula. The contents of flavonoids as well as chemical elements differed in all examined samples and the differences were caused by number of agents such as site and weather conditions of vegetation and covers of the capitula. The inflorescences of red capitulum covers contained more flavonoids comparing to the one of yellow covers. The contents of chemical elements differed as well and the average contents of Mg, K, Mn, Cu and Zn was bigger for the red capitulum covers in the samples picked in 1991. However, the average content of Fe was higher for the inflorescences having yellow covers. The obtained results suggest that the material for medical purposes obtained from plants of different colour of the inflorescence may have different therapeutic properties. P36VARIATION OFCARDENOUDE GLYCOSIDES CONTENT IN HERBA CONVALLARIAE I, Kesinski Deparlmsetof PharmaceuU~ Bot~my, MedicalAcademy of C¢llm,k,80-416Gdansk,Pobnd The results ofbiometric investigations of Herba Convallaria maialis are presented with regard to cardenolide glycosides content in Herba Convallariae as a dependence of the site condition. Examinations have been performed on 22 plots established in the com- munities representing four forest complex of Gdansk Pomerania region. Abundance of population, some of biometrics properties of Convallaria maialis shoot, the total content of cardenolide glycosides have been examined for three years and the changes of these quantities have been observed at the plots due to picking the herbs. The site characteristic was evaluated for each plot of observation, i.e the forest complex was determined for each plot of observation, illumination of the ground cover vegetation and soil pH. The glycoside content was estimated by the method worked out by Strzelecka and her co-workers. The total cardenolide glycoside content varied from 0.038% to 0.131% for the samples collected at 22 plots of observation.