Vol. 12(26), pp. 382-388, 22 September, 2018 DOI: 10.5897/AJPP2016.4698 Article Number: 8CA817758570 ISSN: 1996-0816 Copyright ©2018 Author(s) retain the copyright of this article http://www.academicjournals.org/AJPP African Journal of Pharmacy and Pharmacology Full Length Research Paper Antibacterial activity of leaf and leaf callus extracts of Anisochilus carnosus (L) Wall. Nissar Ahmad Reshi 1* , Sudarshana Mysore Shankarasingh 2 and Girish Vasanaika Hodiyala 2 1 Department of Botany, School of Science, Sandip University, Nashik, Maharashtra, India 2 Department of Studies in Botany, University of Mysore, Mysuru, Karnataka, India. Received 9 November, 2016; Accepted 30 January, 2017 The present study was carried out to determine the antibacterial efficacy of chloroform, petroleum ether, ethyl acetate, methanol, ethanol and aqueous extracts of leaf and leaf derived callus of Anisochilus carnosus against Bacillus cereus, Bacillus subtiltis, Staphylococcus aureus, Streptococcus pyogenes, Vibrio parahaemolytics, Enterobater aerogenes, Proteus mirabilis, and Klebseilla pneumoniae. The leaf segments were cut into small pieces of size 1 sq.cm and were cultured on Murashige and Skoog solid medium supplemented with different auxins alone and in combination. Antibacterial efficacy was performed by disc diffusion method followed by minimum inhibitory concentration (MIC) determination by two fold serial dilution method. Leaf and leaf callus extracts were subjected to the qualitative phytochemical analysis. Maximum callus formation percentage was obtained from the leaf segments cultured on MS medium supplemented with 2, 4-D (3 mg/l). Methanolic leaf extract showed maximum antibacterial activity with 26 mm zone of inhibition against K. pneumonia with MIC value of 0.64 mg/ml. Out of the callus extracts, ethanolic callus extract showed the maximum bioefficacy against K. pneumonia with 20 mm zone of inhibition and MIC value of 1.25 mg/ml. Results revealed that both leaf and leaf derived callus are effective against K. pneumoniae. The bioefficacy study confirms the antibacterial potential of leaf and leaf derived callus of A. carnosus. The study paves a way for the production of secondary metabolites by establishing high potential cell lines of callus tissues. Key words: Leaf, leaf callus, antibacterial efficacy, minimum inhibitory concentration (MIC), Anisochilus carnosus. INTRODUCTION Plants as a source of medicine have been inherited and form the backbone of health care system. From last few decades, there has been surge in the use of natural remedies and now a days traditional medicinal plants have become the focus of study in terms of validation of their traditional uses. Popular observation and scientific validation of medicinal plants will significantly contribute to the development of natural and novel drug system. In India, medicinal plants constitute 50% of the higher flowering plant species (Johnson et al., 2011). Till *Corresponding author. E-mail: nissarreshi@gmail.com. Author(s) agree that this article remain permanently open access under the terms of the Creative Commons Attribution License 4.0 International License