Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Sun, 04 Nov 2018 23:01:54 Streptomyces osmaniensis sp. nov., isolated from soil T. V. K. Reddy, 1 Shaik Mahmood, 1 M. Mohammed Idris 2 and Slawomir Ciesielski 3 Correspondence Shaik Mahmood skmahmood@osmania.ac.in T. V. K. Reddy reddykishoretv@gmail.com 1 Microbiology Lab, Department of Botany, University College of Science, Osmania University, Hyderabad 500 007, India 2 Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India 3 Department of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, ul. Sloneczna 45G, Olsztyn, Poland A novel actinomycete, designated strain OU-63 T , was isolated from garden soil collected on the campus of Osmania University in Hyderabad, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Streptomyces, and was related most closely to Streptomyces chartreusis NBRC 12753 T (98.6 % similarity). However, the mean level of DNA–DNA relatedness between the two strains was only 25±1.73 %. Based on DNA–DNA relatedness, morphological and phenotypic data, strain OU-63 T could be distinguished from the type strains of phylogenetically related species. It is therefore considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces osmaniensis sp. nov. is proposed. The type strain is OU-63 T (5CCTCC AA209025 T 5PCM 2690 T ). The family Streptomycetaceae, which comprises the genera Streptomyces, Kitasatospora and Streptacidiphilus, was proposed by Waksman & Henrici (1943) and its descrip- tion was emended by Kim et al. (2003). At the time of writing, the genus Streptomyces comprises over 550 recognized species (Euze ´by, 2009). Streptomyces species are aerobic actinomycetes and most of them are able to form an extensively branched substrate mycelium and aerial hyphae that typically differentiate into chains of spores. Members of the genus have LL-diaminopimelic acid in the cell-wall peptidoglycan, but no characteristic sugars (wall chemotype I sensu Lechevalier & Lechevalier, 1970) and possess DNA rich in G+C (Williams et al., 1983; Manfio et al., 1995). The streptomycetes, producers of more than half of the 10 000 documented bioactive compounds (for example antibiotics, enzymes, inhibitors and pharmacologically active agents), have offered over 50 years of interest to industry and academia (Anderson & Wellington, 2001; Berdy, 2005). In the course of a screening programme for antibiotics, a novel strain, OU- 63 T , was isolated from a garden soil sample collected on Osmania University campus, Hyderabad, India, and was provisionally assigned to the genus Streptomyces by using chemotaxonomic and morphological properties. A poly- phasic taxonomic investigation based on a combination of phenotypic and genotypic characteristics revealed that strain OU-63 T represents a novel species of the genus Streptomyces. Strain OU-63 T was isolated by using a modification of the protocol developed by Atalan et al. (2000) on actinomycetes isolation agar (Himedia) supplemented with cycloheximide (50 mg ml 21 ) and nystatin (25 mg ml 21 ). The agar was seeded with the soil suspension and incubated for 14 days at 28 u C (Olson, 1968). The new isolate was maintained on oatmeal agar slants [International Streptomyces Project (ISP) medium 3; Shirling & Gottlieb, 1966] at 4 u C and as glycerol suspensions (20 %, v/v) at 220 u C. The morphological characteristics of strain OU-63 T were examined by light and scanning electron microscopy of 14- day-old cultures on ISP medium 3 (Williams & Davies, 1967). The coverslip technique (Zhou et al., 1998; Kawato & Shinobu, 1959) was used to observe hyphae and spore chains by light microscopy. Spore-chain morphology and spore- surface ornamentation were studied by examining gold- coated, dehydrated specimens with a scanning electron microscope (JSM 5600; JEOL). Growth and cultural characteristics were observed on various media after incubation at 28 uC for 14 days. A range of physiological The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain OU-63 T is FJ613126. A table detailing the growth and cultural characteristics of strain OU-63 T on various media is available as supplementary material with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2010), 60, 1755–1759 DOI 10.1099/ijs.0.017749-0 017749 G 2010 IUMS Printed in Great Britain 1755