Vol. 10 | No. 2 |305 -312 | April - June | 2017 ISSN: 0974-1496 | e-ISSN: 0976-0083 | CODEN: RJCABP http://www.rasayanjournal.com http://www.rasayanjournal.co.in Rasayan J. Chem., 10(2), 305 -312(2017) http://dx.doi.org/10.7324/RJC.2017.1021631 ASSESSMENT OF HEPATOPROTECTIVE ROLE OF PHENOLIC EXTRACT OF URTICA DIOICA AND SILVER NANOPARTICLES IN MALE RAT INDUCED BY CARBON TETRA-CHLORIDE M. M. Kadhim 1,* , A.N. Aldujaili 2 and M.H. Homady 3 1 Technical Institute of Diwaniyah/AL-Furat AL-Awsat Technical University, Najaf, Iraq 2,3 Department of Biology, College of Science/University of Kufa, Najaf, Iraq *E-mail: murtadham.alkhafaji@student.uokufa.edu.iq ABSTRACT This study showed the protective role of the phenolic extract of Urtica dioica and silver nanoparticles (AgNPS) on the hepatotoxicity effect via carbon tetrachloride (CCL4) within white male rats. Hundred and ten male albino rats weighing (200-250g) and aged (10-17week). Animals were housed in the animal house of faculty of Science/University of Kufa under control condition; light 12 and 12 dark hours and temperature (24±2C°).Animals divided into four groups as follows the control groups, carbon tetrachloride groups, phenolic extract groups, and silver nanoparticles, groups. Subdivided groups above depending on the concentration (250 and 500mg/kg) of phenolic extract, (10 and 5mg/kg) of silver nanoparticles for (two and three) month add to dosage after and before induced by carbon tetrachloride (five animals per group).The current study revealed the occurrence of a significant decrease (P≤0.05) in the level of regucalcin when the administered by phenolic extract and silver nanoparticles for (two and three months) compared with carbon tetrachloride group. Highly significant (p≤0.05) with the concentration of the (500mg/kg) for phenolic extract and (10mg/kg) for silver nanoparticles, and no significant observed between extract and silver nanoparticles for (two and three months) and before and after induced by the carbon tetrachloride. Keywords: Carbon tetrachloride, Hepatotoxicity, Urtica dioica, Silver nano-particles, Regucalcin. © RASĀYAN. All rights reserved INTRODUCTION Liver occupies the first degree in the most management the important of human body, so it is the largest organ of the achievement of important functions and is one of the main focus for construction and secretion and contribute to the metabolism and storage operations for the liver importance in most homeostasis processes and energy saving, as well as the production of bile 1 . The detoxification of the greatest pivotal functions of the body being responsible for the process of converting and removing harmful substances 2 . Carbon tetrachloride is extensively employed for initiation of liver injury. 3 Carbon tetrachloride has been widely used as a model of toxicity-induced chronic liver injury, progressing to fibrosis and cirrhosis 4,5 . The belief causes of carbon tetrachloride are provoked hepatic destruction in lipid peroxidation and decline action of antioxidant enzymes and accumulation of free radicals. 6,7 Urtica dioica L. herbaceous plant that grows over a wide area of the world belongs to the family Urticaceae and includes more than sixty genera and about more than seven hundred species the commonly known as Stinging Nettle. 8,9 The diskette leaves rich in fine hair, which irritates the skin on contact because they contain many materials including acetylcholine, histamine. 10,11 Previous studies have shown phytochemical compounds as flavonoids, tannins, volatile compounds and sterols. 9,12,13 Common uses of U. dioica in the treatment of arthritis and rheumatic add to the diuretic agent. 14 Utilised U. dioica extract to the prevention of hepatic and renal diseases because of the functions which are performed by such activate antioxidants system and destroy free radicals in addition to protective in the hepatic ischemia-reperfusion injury in rats. 15–18