           !"  Tanakiat Chompupong a , Florian T. Schevenels b , Thanaset Senawong c , and Ratsami Lekphrom a * a Natural Products Research Unit, Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand b Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand c Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand #: Phytochemical investigation of the flowers of Anomianthus dulcis led to the isolation of eleven known compounds. The isolated compounds were identified by analysis of physical and spectroscopic (IR, 1D and 2D NMR) data. We report five flavonoids ($%& and $’), two chalcone,sesquiterpenoids (( and )), one indole alkaloid (*), one triterpenoid (+), and a mixture of two phenolic compounds (, and $$). Remarkably, it is the second isolation of sumadain A (() and sumadain B ()) from a natural source. Compounds ( and ) were evaluated against several cancer cell lines, as the previous report lacked biological activity studies. Compound ) showed strong cytotoxicity against Hela, HT,29, HCT,116, and A549 cell lines, with IC 50 values in the range of 5.43–14.64 g/mL. -./: Anomianthus dulcis, Annonaceae, sumadain, anti,proliferative activity *Corresponding author E,mail address: ratsami@kku.ac.th