Int.J.Curr.Microbiol.App.Sci (2018) 7(10): 3524-3531 3524 Original Research Article https://doi.org/10.20546/ijcmas.2018.710.407 Two-Dimensional Gel Electrophoresis of Protein profile from Groundnut (Arachis hypogaea sp. Virginia) at Flower Development Stage Nimita Umretiya 1* , Hitesh Jasani 2 , Nilam Tulsani 1 , Abhijeeta Nandha 1 and B.A. Golakiya 1 1 Department of Biotechnology, Junagadh Agricultural University, Junagadh-362001, Gujarat, India 2 College of Computer, Science and Information Technology, Junagadh-362015, Gujarat, India *Corresponding author ABSTRACT Introduction The most precarious step in any proteomics study is sample extraction and preparation. In this regard, proteomic analysis of plant tissues involves a number of practical challenges that are typically more problematic than with other samples. In addition to having relatively low protein concentrations, plant tissues are often higher in proteases enzyme and materials that severely restrict with downstream protein purification and analysis, including cell wall and storage polysaccharides, phenolic mixtures, lipids and a wide-ranging of secondary metabolites (Granier, 1988; Tsugita and Kamo, 1999). Groundnut or peanut (Arachis hypogaea L.) is one of the principal economic crops of the world (Cobb and Johnson, 1973). It is most popular and universal legume crops, cultivated in more than 100 countries. Groundnut plays an important role in the agricultural and industrial economy of the country. Arachis Protein is play critical role in flower development. Profiles of total flower development proteins isolated from different stages (Bud, Flower and Peg) peanut cultivar GJG-22, were studied using two-dimensional gel electrophoresis. Protein is extracted by trichloro acetic acid/acetone precipitation method. Comparative analysis was carried out of spots identified using platinum master software at three different flower stages. Protein revealed that total 9193 protein spots with the pH range 4 - 7 and 23-312 kDa range recorded. Out of 9193 spots, maximum 3413 spots were found in pH range 6 - 7 in groundnut samples. Proteomic analysis reveals the translational products of gene expression of plant during flowering stage. Keywords Two-Dimensional Electrophoresis (2- DE), Protein profile, Groundnut, Arachis hypogaea, Protein extraction Accepted: 24 September 2018 Available Online: 10 October 2018 Article Info International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 10 (2018) Journal homepage: http://www.ijcmas.com