Contents lists available at ScienceDirect Marine Genomics journal homepage: www.elsevier.com/locate/margen The complete genome of the oil emulsifying strain Thalassolituus oleivorans K-188 from the Barents Sea Victoria Yu Shtratnikova a,⁎ , Ilya Belalov b , Artem S. Kasianov c , Mikhail I. Schelkunov a,d , D. a Logacheva Maria a , Andrey D. Novikov e , Alexey A. Shatalov e , Tatyana V. Gerasimova e , Alexander S. Yanenko e , Vsevolod J. Makeev c,e,f a A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskye gory, h. 1, b. 40, Moscow 119991, Russian Federation b Winogradsky Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave., h. 33, b. 2, Moscow 119071, Russian Federation c Vavilov Institute of General Genetics, Gubkina str., h. 3, Moscow 119991, Russian Federation d Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoy Karetny per., h. 19, b. 1, Moscow 127051, Russian Federation e State Institute for Genetics and Selection of Industrial Microorganisms, 1-st Dorozhniy pr., h. 1, Moscow 117545, Russian Federation f Moscow Institute of Physics and Technology, 9 Institutskiy per., Dolgoprudny, Moscow Region 141700, Russian Federation ARTICLE INFO Keywords: Thalassolituus oleivorans K-188 Complete genome sequencing Oil emulsifier Polar sea environment ABSTRACT Gammaproteobacterium Thalassolituus oleivorans plays an important role in oil degradation in sea water through emulsifying crude oil and alkanes at low temperatures in polar sea environment. Here we report the complete genome sequence of K-188 strain (VKPM B-9394) isolated in the Barents Sea and compare it with other known Thalassolituus oleivorans strains. The Thalassolituus strains are differed in orthologs number of the genes of alkane degradation, transport proteins, genes of sugar utilization, endonucleases, signaling proteins, transcriptional regulators and presence of CRISPR/Cas locus. Also only the genome of K-188 contains the 3-hydroxyalkanoate synthetase. 1. Introduction Hundreds of species can use hydrocarbons as a primary source of carbon (Hazen et al., 2016). Oceanospirilalles are dominant species in the initial phase of the oil spill degradation (Hazen et al., 2016). This group includes such well-known hydrocarbon degraders and surfactant producers as Alcanivorax borkumensis (Kasai et al., 2002; Yakimov et al., 1998), Thalassolituus oleivorans (Golyshin et al., 2013), Oleispira ant- arctica (Kube et al., 2013). The chemistry and enzymology of oil me- tabolism in sea waters has been studied for the most part in tropical waters (Teramoto et al., 2009). Of the two T. oleivorans strains for which complete genome sequences are available, one was isolated in the warm Mediterranean environment (MIL-1, (Golyshin et al., 2013)), the other in the extremely cold Chukchi Sea (R6-15, (Dong et al., 2014)). The sequencing of a third (polar) strain would allow studying traits related to cold water environment. T. oleivorans K-188 (Russian National Collection of Industrial Microorganisms, VKPM B-9394) strain was isolated by A. Novikov and A. Shatalov during their Barents Sea expedition. When cultivated on n- alkanes this strain produces extracellular biosurfactant and, at low temperature, also emulsifies crude oil (Shatalov et al., 2005). Cold water adaptation allows Thalassolituus to thrive in polar surface waters and probably in deep waters around the world (Hazen et al., 2016). In this communication we present the complete genome of T. oleivorans K- 188 strain, and compare the composition of important gene families in three T. oleivorans strains. 2. Data description Two library types were prepared for Illumina sequencing. The paired end library (with 300 bp insert) was prepared with TruSeq DNA Sample Preparation (Illumina) kit after fragmenting of genomic DNA with Covaris S220 and sequenced on HiSeq 2000 yielding paired end 100 bp reads (PE). Mate-pair libraries with 2000—8000-bp-insert fragments were created with Nextera mate-pair sample preparation kit (Illumina), using gel-free library preparation, and sequenced on MiSeq http://dx.doi.org/10.1016/j.margen.2017.08.005 Received 31 July 2017; Accepted 17 August 2017 ⁎ Corresponding author. E-mail addresses: vtosha@yandex.ru (V.Y. Shtratnikova), ilya.belalov@gmail.com (I. Belalov), artem.kasianov@gmail.com (A.S. Kasianov), shelkmike@gmail.com (M.I. Schelkunov), maria.log@gmail.com (D.,. Logacheva Maria), andrnov@genetika.ru (A.D. Novikov), alshatalov@gmail.com (A.A. Shatalov), yanenko@genetika.ru (A.S. Yanenko), vsevolod.makeev@gmail.com (V.J. Makeev). Abbreviations: MP, mate-pair (reads or library); PE, Short paired-end (reads or library); CRISPR/Cas, Clustered regularly interspaced short palindromic repeats Marine Genomics xxx (xxxx) xxx–xxx 1874-7787/ © 2017 Elsevier B.V. All rights reserved. Please cite this article as: Shtratnikova, V.Y., Marine Genomics (2017), http://dx.doi.org/10.1016/j.margen.2017.08.005