Open Peer Review Discuss this article (0) Comments RESEARCH NOTE Characterization and stability evaluation of nanoencapsulated epoxylignans [version 1; referees: 1 approved, 1 approved with reservations] Yusnita Rifai , Radhia Riski , Gemini Alam , Magdalena Litaay , Latifah Rahman 1 Pharmaceutical Chemistry Laboratory, Faculty of Pharmacy, Hasanuddin University, Makassar, 90245, Indonesia Biology Department, Faculty of Life Sciences, Hasanuddin University, Makassar, 90245, Indonesia Abstract 3',6-dimethoxy-3'',4''-(methylenedioxy)-2,5-epoxylignan-4'-ol (DMEO), an epoxylignan isolated from , has currently captured attention for its Piper nigrum potential antitumor effect. However, low stability is limiting its therapeutic application. The application of nanocapsulation would be the main strategy for overcoming this problem. DMEO-loaded nanocapsules were prepared by an emulsion-diffusion method using Eudragit RL 100 (at concentrations of 1, 1.5 and 2%) and polyvinyl alcohol. As the polymer content increased, the encapsulation efficiency and mean particle size also increased. After 6 months of storage at 25°C (0% RH), no crystalline peaks were observed in the diffraction patterns of all nanocapsules, thereby suggested that the physical stability of nanoencapsulated DMEO was not affected by the concentration ratio of the polymer−stabilizer combinations. Keywords nanocapsules, Eudragit RL 100, Glioma, Piper nigrum 1 1 1 2 1 1 2 Referee Status: Invited Referees version 2 published 30 Jul 2018 version 1 published 01 Mar 2018 1 2 report report , Universitas Indonesia, Rani Sauriasari Indonesia 1 , Bandung Institute of Heni Rachmawati Technology, Indonesia 2 01 Mar 2018, :253 (doi: ) First published: 7 10.12688/f1000research.13047.1 30 Jul 2018, :253 (doi: ) Latest published: 7 10.12688/f1000research.13047.2 v1 Page 1 of 9 F1000Research 2018, 7:253 Last updated: 01 AUG 2018