ABSTRACT The hemolytic activity process was applied to erythrocytes by an analyst, as well as the lysis of erythrocytes resulted in a positive test result. The L. aucheri Boiss. extracts are high in triterpenoids saponins, according to the fndings. Both extracts were used in acute toxicity in vivo research on four groups of rats (six rats in each group). After 72 hours of treatment with various concentrations of terpenoids saponins extracts (25, 50, and 100 mg/kg BW), no mortality was detected in the rats of the trials. This demonstrated that both extracts are harmless when taken orally. Keywords: Acute toxicity, Blood Hemolytic, Extraction, L. aucheri Boiss. International Journal of Drug Delivery Technology (2022); DOI: 10.25258/ijddt.12.2.73 How to cite this article: Sultan MQ, Kredy HM, Charfeddine B. Blood Hemolytic Activity and Acute Toxicity of Saponins extract from Lepudium aucheri Boiss. International Journal of Drug Delivery Technology. 2022;12(2):870-872. Source of support: Nil. Confict of interest: None Blood Hemolytic Activity and Acute Toxicity of Saponins extract from Lepudium aucheri Boiss. Mohammed Q. Sultan 1 *, Husam M. Kredy 2 , Bassem Charfeddine 3 1 College of Dentistry, Al–Ayen University, Thi-Qar, Iraq. 2 College of sciences, University of Thi-Qar, Iraq 3 Faculty of Medicine, Department of Biochemistry, University of Sousse, Tunisia Received: 25th February, 2022; Revised: 24th April, 2022; Accepted: 07th May, 2022; Available Online: 25th June, 2022 INTRODUCTION Hemolysis occurs when the structure of erythrocyte cell membranes is damaged or disrupted, causing hemoglobin to be released. Hemolysis of red blood cells happens during the assembly of the components, storage, distribution, and delivery to the patient. The percentage of free hemoglobin in the medium used to suspend red blood cells, such as a plasma or added solutions., indicates hemolysis in blood products. Storage of erythrocytes leads to a high level of hemolysis. Various procedures, most popular, which is visual assessment, can be used to quantify the amount of hemolysis. Spectrophotometric tests, photometric methods, and microplate technology are among the others. 1 Two diferent methods cause hemolysis. Intravascular hemolysis occurs when red blood cells are destroyed in the circulation, and their contents are released into the plasma. Mechanical damage from a weakened endothelium can trigger direct membrane breakage and cell death, Infectious microorganisms, as well as complement attachment and activation on the cell surface Extravascular hemolysis is the removal and destruction of erythrocytes by spleen and liver macrophages with cell wall alterations. 2 MATERIAL AND METHOD Plant Collection Plant collected in March 2013 from local markets in Nasiriyah city at, Iraq. RESEARCH ARTICLE Extraction of Crude Saponin from a Plant Air-dried powdered plants of Lepidium aucheri boiss (100 g) were separated with 70% ethanol (24 hrs x 1000 mL) at room temperature. By low pressure evaporated at 45°C, the organic phase was concentrated to a small amount (300 mL) and isolated in three steps with chloroform (24 hrs x 100 mL x 3) and n-Butanol (24 hrs x 100 mL x 3). Given the saponins extract, the n-Butanol layer was reduced to dried (10 g). Saponins Compounds are Tested using the Following Method • Aqueous saponin extract solution was made in a test tube and shaken, after which the soapsuds remained for a long period. • 5 mL saponin extract aqueous solution in test tube, 5 mL silver nitrate, then placed in a water bath at 100°C for 5 minutes, resulting in a silver mirror on the inner surface of the test tube 15. 3 Acute Toxicity Study for Saponins Extracts Laboratory animals were separated into four groups, each with six rats, according to the following: Group 1: normal controls who were given 0.2 mL of DMSO Group 2: Rats were given Saponins extract (25 mg/kg). Group 3: Rats were given Saponins extract (50 mg/kg). Group 4: Rats were given Saponins extract (100 mg/kg). In acute toxicity testing, only water was available to the animals throughout their four-hour fast. Saponins extract was *Author for Correspondence: mohammed.alkhuzaie@alayen.edu.iq