Int.J.Curr.Microbiol.App.Sci (2014) 3(2): 475-479 475 Original Research Article Bioactivity of stigmasterol isolated from the aerial part of Spillanthes acmella (Murr) on selected microorganism Isah Yinusa 1* , Ndukwe Iloegbulem George 2 , Usman O.A. Shuaibu 1 and R.G.Ayo 3 1 Department of Chemistry, Federal University, Lokoja, Kogi state, Nigeria 2 Department of Chemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria 3 Samaru College of Agriculture, Ahmadu Bello University, Zaria, Kaduna State, Nigeria *Corresponding author ABSTRACT Introduction Spillanthes acmella Murr is a flowering herb in the family Asteraceae. It is known as toothache plant or pancress as the leaves and the flower heads contain analgesic agent Spilanthol that is used to numb toothache. The fresh and cooked leaves are combined with chilies and garlic to add flavour and vitamins to foods (Miyazawa et al; 2006). Some chemical constituents have been isolated from the plant such as Spilanthol an alkyl amide (Gokhale and Bhide, 1945). This is responsible for the saliva inducing effect of the plant, and some triterpenoids (Ramsewak et al; 1999). The leaves along with alum are recommended as emetic (Gill, 1992). Because of this observation and the regular uses of the plant by the natives prompted this research possibly to find out if there could be more metabolites that can be of pharmacological relevance. ISSN: 2319-7706 Volume 3 Number 2 (2014) pp. 475-479 http://www.ijcmas.com Keywords Bioactivity; Stigmasterol; Spillanthes; antimicrobial; inhibition; MIC. The Stigmasterol was isolated from Spillanthes acmella Murr previously, which was subjected to antimicrobial activity test using the following clinical microbes to determine its biological activities; Methycilin Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Streptococcus pyrogenes, Bacillus subtilis, Corynebacterium ulcerans, Escherichia coli, proteus mirabilis, proteus vulgaris, Pseudomonas aureginosa, Salmonella typhi, Shigellia dysenteriae, Candida albicans, Candida virusei, and Candida tropicalis. The zone of inhibition was observed to be between 20 to 24 mm with Bacillus Subtilis having the largest zone of inhibition of 24 ml.The Minimum Inhibitory Concentration (MIC) and The Minimum Fungicidal Concentration (MFC) was determined. It was found that the MFC was higher than that of standard drug Fluconazole. This compound has a wide range of broad spectrum over the standard drug against the entire test organism.