Volume 8, Issue 5, May – 2023 International Journal of Innovative Science and Research Technology ISSN No:-2456-2165 IJISRT23MAY1830 www.ijisrt.com 2824 Increase of Seed Vigor Local Upland Rice Kambowa Cultivar Using Combination Endo-Rhizobacteria Zainal Abidin 1 1 Plantation Plant Cultivation Study Program Samarinda State Agricultural Polytechnic Samarinda, Indonesia F. Silvi Dwi Mentari 1 1 Plantation Plant Cultivation Study Program Samarinda State Agricultural Polytechnic Samarinda, Indonesia Riama Rita Manullang 1 1 Plantation Plant Cultivation Study Program Samarinda State Agricultural Polytechnic Samarinda, Indonesia Yuanita 1 1 Plantation Plant Cultivation Study Program Samarinda State Agricultural Polytechnic Samarinda, Indonesia Haryatie Sarie 2 2 Environmental Management Study Program Samarinda State Agricultural Polytechnic Samarinda, Indonesia Daryono 1 1 Plantation Plant Cultivation Study Program Samarinda State Agricultural Polytechnic Samarinda, Indonesia La Mudi 1* 1 Plantation Plant Cultivation Study Program, Samarinda State Agricultural Polytechnic Samarinda, Indonesia Abstract:- Many agricultural development technologies to increase national rice production has been carried out, one of which is the use of biological agents. Single-use of biological agents has been widely reported to increase rice production. To optimize national rice production, a combination of biological agents is applied so that rice production is expected to increase even higher. This research aimed to increase the seed vigor of the Kambowa upland rice cultivar using a combination of endo-rhizobacteria. This research was carried out at the Faculty of Agriculture, Halu Oleo University, Laboratory of Agronomy, in April 2018. The study used a completely randomized design, with 5 treatments, namely: isolates Be02 + PKLK, isolates Be02 + Bacillus sp. CKD061, isolates PKLK5 + Bacillus sp. CKD061 and isolates Be02 + PKLK5 + Bacillus sp. CKD061. Each treatment was repeated 3 times to obtain 15 experimental units. Variables observed included seed germination, vigor index, maximum growth potential, growth uniformity, relative growth rate, T50, and root length. The research data were analyzed using analysis of variance and continued with the DMRTα= 0.05 test. The results showed that the combination treatment of endo-rhizobacteria increased the seed vigor of the Kambowa cultivar upland rice. Combination treatments of endo-rhizobacteria isolate Be02 + isolate PKLK5 increased germination, seed vigor index, maximum growth potential, growth uniformity, and root length. Combination treatment of endo-rhizobacteria isolates Be02 + PKLK5 + Bacillus sp. CKD061 resulted in a relative growth rate and treatment of isolates Be02 + Bacillus sp. CKD061 gave the best T50 compared to the control. Keywords:- IAA; Isolated PKLK5; Isolated Be02; Local Upland Rice. I. INTRODUCTION Rice is an important role in Indonesia because it is the major source of food for the people. The rice in Indonesia consists of various types of diversity, one of which includes upland rice. Upland rice is a local type of rice that is greatly cultivated by local people as a staple food, especially in rural areas. The availability of seed quality is the main obstacle to the upland rice cultivation system. In the past, farmer use has only used seeds obtained from other farmers or stored seeds from previous plantings with unsecured quality the seed and can result in low seed vigor. Increased seed vigor can be done by providing seed treatment through seed invigoration by utilizing environmentally friendly biological agents that can act as plant growth promotion. Endophytic bacteria are biological agents in the form of microorganisms that can live and associate in tissues of the host plant from seed germination to advanced development that do not cause negative impacts so they can provide benefits to the host plant [1] [2]. Single-use of biological agents has been widely reported can increase plant seed germination [3] and at the same time can stimulate plant growth [4], and can increase plant resistance to pathogens