Citation: Go´ zdziuk, M.; Kavetskyy, T.; Roquero, D.M.; Smutok, O.; Gonchar, M.; Královiˇ c, D.P.; Švajdlenková, H.; Šauša, O.; Kalinay, P.; Nosrati, H.; et al. UV-Cured Green Polymers for Biosensorics: Correlation of Operational Parameters of Highly Sensitive Biosensors with Nano-Volumes and Adsorption Properties. Materials 2022, 15, 6607. https://doi.org/10.3390/ ma15196607 Academic Editor: Béla Iván Received: 30 August 2022 Accepted: 16 September 2022 Published: 23 September 2022 Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affil- iations. Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). materials Article UV-Cured Green Polymers for Biosensorics: Correlation of Operational Parameters of Highly Sensitive Biosensors with Nano-Volumes and Adsorption Properties Magdalena Go ´ zdziuk 1 , Taras Kavetskyy 2,3, * , Daniel Massana Roquero 4 , Oleh Smutok 4,5 , Mykhailo Gonchar 2,5 , David P. Královiˇ c 6 , Helena Švajdlenková 7 , Ondrej Šauša 6,8 , Pavol Kalinay 8 , Hamed Nosrati 9 , Migle Lebedevaite 10 , Sigita Grauzeliene 10 , Jolita Ostrauskaite 10 , Arnold Kiv 11 and Bo ˙ zena Zgardzi ´ nska 1, * 1 Institute of Physics, Maria Curie-Sklodowska University, 20-031 Lublin, Poland 2 Department of Biology and Chemistry, Drohobych Ivan Franko State Pedagogical University, 82100 Drohobych, Ukraine 3 Department of Materials Engineering, The John Paul II Catholic University of Lublin, 20-950 Lublin, Poland 4 Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA 5 Department of Analytical Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine 6 Department of Nuclear Chemistry, Comenius University in Bratislava, 84215 Bratislava, Slovakia 7 Polymer Institute, Slovak Academy of Sciences, 84541 Bratislava, Slovakia 8 Institute of Physics, Slovak Academy of Sciences, 84511 Bratislava, Slovakia 9 Department of Pharmaceutical Biomaterials, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan 45139-56111, Iran 10 Department of Polymer Chemistry and Technology, Kaunas University of Technology, 50254 Kaunas, Lithuania 11 Department of Innovation Technologies, South-Ukrainian K.D. Ushynsky National Pedagogical University, 65020 Odesa, Ukraine * Correspondence: kavetskyy@yahoo.com (T.K.); bozena.zgardzinska@mail.umcs.pl (B.Z.); Tel.: +38-03244-37700 (T.K.); +48-81-537-6286 (B.Z.) Abstract: The investigated polymeric matrixes consisted of epoxidized linseed oil (ELO), acrylated epoxidized soybean oil (AESO), trimethylolpropane triglycidyl ether (RD1), vanillin dimethacrylate (VDM), triarylsulfonium hexafluorophosphate salts (PI), and 2,2-dimethoxy-2-phenylacetophenone (DMPA). Linseed oil-based (ELO/PI, ELO/10RD1/PI) and soybean oil-based (AESO/VDM, AESO/VDM/DMPA) polymers were obtained by cationic and radical photopolymerization reactions, respectively. In order to improve the cross-linking density of the resulting polymers, 10 mol.% of RD1 was used as a reactive diluent in the cationic photopolymerization of ELO. In parallel, VDM was used as a plasticizer in AESO radical photopolymerization reactions. Positron annihilation lifetime spectroscopy (PALS) was used to characterize vegetable oil-based UV-cured polymers regarding their structural stability in a wide range of temperatures (120–320 K) and humidity. The polymers were used as laccase immobilization matrixes for the construction of amperometric biosensors. A direct dependence of the main operational parameters of the biosensors and microscopical characteristics of polymer matrixes (mostly on the size of free volumes and water content) was established. The biosensors are intended for the detection of trace water pollution with xenobiotics, carcinogenic substances with a very negative impact on human health. These findings will allow better predictions for novel polymers as immobilization matrixes for biosensing or biotechnology applications. Keywords: vegetable oil-based polymers; UV curing; positron annihilation; immobilization matrixes; amperometric biosensors Materials 2022, 15, 6607. https://doi.org/10.3390/ma15196607 https://www.mdpi.com/journal/materials