WOOD RESEARCH doi.org/10.37763/wr.1336-4561/66.4.657665 e-ISSN 2729-8906 66(4): 2021 657-665 pp. 657 AGRO-FORESTRY RESIDUES VALORIZATION BY LIGNINOSOME OF GRIFOLA FRONDOSA MILICA GALIĆ, JASMINA ĆILERDŽIĆ, JELENA VUKOJEVIĆ, MIRJANA STAJIĆ UNIVERSITY OF BELGRADE SERBIA (RECEIVED SEPTEMBER 2020) ABSTRACT Grifola frondosa HAI 1232 was tested for ligninolytic enzyme activities and for lignin, cellulose and hemicellulose degradation during cultivation on eight common agro-forestry residues in Serbia. Wheat straw was favorable lignocellulosic for the production of Mn-dependent and Mn-independent peroxidases (2513.89 and 354.17 U L -1 , respectively), while selected residues inhibited the synthesis of laccases. The highest lignin removal was observed during fermentation of blackberry sawdust (36.75%), while the highest selectivity index was recorded on oak sawdust (4.34). The dry matter loss varied between 8.17% in corn stalks and 14.16% in apple sawdust. According to the presented results, it can be concluded that G. frondosa HAI 1232 could be an important participant in various biotechnological processes due to its high capacity to selectively degrade different agro-forestry residues. KEYWORDS: Agro-forestry residues, delignification, Grifola frondosa, ligninolytic enzymes. INTRODUCTION In the future bio-based society, global challenges such as climate changes, ecosystem degradation, associated with industrialization and growing global population, force us to find effective biological solutions. Fungi are relatively understudied, but according to Hyde et al. (2019) they are an essential and biotechnologically useful group of organisms with a remarkable potential for industrial exploitation. It is known that many modern industries are based on fungi and their metabolites and it is believed that these organisms are the future cell factories for the production of food, pharmaceuticals, enzymes and bio-control agents (Goyal et al. 2016, Lange et al. 2020, Meyer et al. 2020). Reducing dependence on non-renewable, unsustainable resources is one of the keys for stable economic growth and sustained environmental quality (Antov et al. 2020). Industrial and