Evaluation of efficacy and toxicity of poly herbal lozenges in experimental animals ISSN (Print) 2521-8514 ISSN (Online) 2521-8484 RADS J. pharm. pharm. sci 22 Open Access Full Length Article Evaluation of Efficacy and Toxicity of Poly Herbal Lozenges in Experimental Animals Hina Rehman* 1 , Zeeshan Ahmed Shaikh 1 , Safila Naveed 2 , Muhammad Ibrar Shinwari 3 , Khan Usmanghani 1, 2 1 Research and Development Department, Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan. 2 Jinnah University for Women, Karachi, Pakistan. 3 Department of Environmental Sciences,Faculty of Basic & Applied Sciences,International Islamic University,Islamabad Pakistan. ABSTRACT Keywords: Poly herbal lozenges, cough, toxicity testing, Efficacy, Lipid profiles, Biochemical toxicity. Author`s Contribution All the authors contributed significantly to the research that resulted in the submitted manuscripts Article info. Received: Feb 13, 2018 Accepted: Mar 25, 2018 Funding Source: Nil Conflict of Interest: Nil Cite this article: Rehman H, Shaikh A, Naveed S, Usmanghani K . Evaluation of efficacy and toxicity of poly herbal lozenges in experimental animals. RADS J. pharm. pharm. sci. 2018;6(1):22-32. Address of Correspondence Author: drhinarehman@hotmail.com Objective: The study focused on efficacy and toxicity of poly herbal Linkus lozenges in experimental animal. Methodology: The Acute toxicity test for Linkus lozenges was performed on Albino rats Strain Haffkine with both Sex ratio 1:1.Weight was between 150 – 250 g and ages were 4 – 6 months.0.21 g / kg is the therapeutic dose of the preparation. The morphological examination of organs and estimation of biochemical parameters were assessed for acute and chronic testing. Recommended adult dose was 15 g/day, Dose /kg of body weight was 0.21 g/kg however the therapeutic dose of the preparation was20 (4g/kg) and was tested for chronic toxicity. Housed condition was 25˚C ± 1˚C and 12 h dark/light cycle and food at labitium. In all animals, the drug was administered orally at the dose of 4 g/kg. The animals were kept for acute and chronic toxicity under observation for 2 month and 48 hrs. Results: No changes in elements of respiratory cardiovascular focal sensory system, gastrointestinal and excretory framework were seen. Average values of different biochemical parameters in test groups were comparable to average values of respective biochemical parameters in control group of animals and found satisfactory however no mortality and abnormality were observed. Conclusion: No mortality and no abnormality were seen in Linkus lozenges and suggested to be the safe choice. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. INTRODUCTION Cough and sore throat are the frequent symptoms of respiratory disorders and usually annoying and exhausting the patients. Cough is a symptom which cleans the foreign materials and mucus from the upper respiratory track. Due to coughing some mucus or phylum come out from mouth, if not treated well it worsens the normal daily life. If not treated well it causes multiple problems including headache, vomiting, chest pain etc. [1].Continuous coughing creates difficulty in speaking and cause discomfits [2]. Literature studies proves that the herbal and complementary medicine provide a treatment for cough and related symptoms with safety [3, 4].The cause of not practicing herbal plants and ORIGNAL ARTICLE