CHARACTERIZATION FROM WILD RICE OF S * Das Vivekananda Institute of Biotechno par ARTICLE INFO ABST Method cultiva collect These i capacit culture laterite dose w for eac rice. Th Copyright © 2021. Das et al. This is an open access distribution, and reproduction in any medium, provided INTRODUCTION Exploration of microbe-based symbiosis in pl effective ways in the development of sustainable a 2014). Bacterial endophytes can colonize ubiqu plant tissues without causing any morphologica symptom in the host plant. Barraquia et al. Philippines, isolated diazotrophs from wetla successfully developed rice – endophytic diazo The endophytes promote plant growth through production of growth promoting substances and plants against phytopathogenesis ( Ryan, 2008) isolated a number of IAA producing endophytic and soybean root and demonstrated their enhan growth. Phetcharat and Duanga paeng (2012) endophytic bacteria from organic rice and among to produce IAA over 10 g per ml. Jasim et al. growth promoter Indole 3-Acetic Acid produc bacteria is induced by the photochemical of host p *Corresponding author: Das, S. K., Vivekananda Institute of Biotechnology, Sri Ramkris P.O. Nimpith Ashram, Dt S 24 parganas, West Bengal In ISSN: 0975-833X Article History: Received xxxxxxxxxxxxxxxxxxxxxxxx Received in revised form xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx Accepted xxxxxxxxxxxxxxxxxxxxxxxxx Published online xxxxxxxxxxxxxxxxxx RESEARCH ARTICLE Citation: Das, S. K., Das, T. K. and Podder, A. K. “C inm package for rice”, 2021. International Journal of Article History: Received 09 th February, 2021 Received in revised form 14 th March, 2021 Accepted 20 th April, 2021 Published online 20 th May, 2021 Key Words: Oryza rufipogon, Endophytic Diazotrophs, Laboratory Studies, Pot Experiment, Field trial, Selection as Biofertilizer. OF ENDOPHYTIC DIAZOTROP SUNDARBANS FOR THEIR INCL PACKAGE FOR RICE as, S. K., Das, T. K. and Podder, A. K. nology, Sri Ramkrishna Ashram Nimpith, P.O. Ni rganas, West Bengal India PIN 743338 TRACT d for selection of endophytic diazotropic isolates for use ation is developed and standerdized. Ten endophytic d ted from roots of a wild rice species, Oryza rufipogon, from isolates were characterised on the basis of nitrogenage activ ty. These isolates were also tested for contribution in yield e. Based on these studies, 4 isolates were selected for field e zones of West Bengal. In the trial 25% ( 20 – 22.5 kg) o was deducted and supplemented by endophytic diazotropic ch zones were selected on the basis of their contribution in 1 hese isolates are recommended for inclusion in INM packag s article distributed under the Creative Commons Attribution Li the original work is properly cited. lants is one of the agriculture (Marella, uitously in the inner al change or disease (1997) from IARI, and rice and also otroph combinations. h nitrogen fixation, d protecting the host ). Yu et al.( 2016) c bacteria from corn ncing effect on crop ) got 26 isolates of g them, 4 were found (2014) reported that ction by endophytic plant. shna Ashram Nimpith, ndia PIN 743338. Siderophores are are small, iron-ch which are secreted by many ba growth promoter as well as biocon reported the production of sidero their potential role in plant growt Pahari et al., 2017; Loaces et al., can form natural association in ro fixing (Diazotrophic) microorgan responsible for supply of fixed nitr growth promotion through o colonization of endophytic diazotr soil in absence of host plant is an i effective microorganism in soil abiotic factors in soil (Olivera e Bashan and Vazquez, 2000). The root exudates in soil, presence o host, presence of organic matter includes, soil texture, soil moisture field. It has been speculated that w population of nitrogen fixing bacte varieties of cultivated rice. Report wild rice harbour qualitatively an diazotrophs than cultivated rice. International Journal of Current Research Vol. 13, Issue, 05, pp.17383-17386, May, 2021 DOI: https://doi.org/10.24941/ijcr.41415.05.2021 I Available online at http://www.journalcra.com Characterization of endophytic diazotrophic bacteria from wild rice f Current Research, 13, (05), 17383-17386. PHIC BACTERIA LUSION IN INM Nimpith Ashram, Dt S 24 se as nitrogen bio-fertilizer in rice diazotropic bacterial isolates were m Sunderban area of West Bengal. vity, siderophore & IAA production attributing parameters in rice in pot d trials in coastal saline and red & of recommended chemical nitrogen bacteria application. Two isolates 1000 grain weight and total yield in ge in rice cultivation. icense, which permits unrestricted use, helating compounds of high affinity acteria and fungi. It acts as plant ntrol agents in soil. Many workers ophore by endophytic bacteria and th promotion (Vendan et al., 2010; 2011). It is known that rice plants oots with various types of nitrogen nisms. These microorganisms are rogen to the rice plant in addition to other activities. For successful rophs in rice roots, their survival in important factor. But the survival of depends upon several biotic and et al., 2004; Bashan et al., 1995; biotic factors includes, presence of of weeds to function as alternative r in soil, etc. The abiotic factors e, pH , geographical location of the wild rice is likely to harbour unique eria that differ from those in modern ts are available to demonstrate that nd quantitatively better endophytic INTERNATIONAL JOURNAL OF CURRENT RESEARCH OPEN ACCESS e of sundarbans for their inclusion in