ew Open Access | Page 120 | Vol 5 | Issue 1 | Pages 120-124 Copyright: © 2021 Hamid OM. This is an open-access artcle distributed under the terms of the Creatve Commons Atributon License, which permits unrestricted use, distributon, and reproducton in any medium, provided the original author and source are credited. Annals of Microbiology and Research ISSN: 2642-4533 SCHOLARS. DIRECT DOI: 10.36959/958/580 *Corresponding author: Magdi A Bayoumi, Faculty of Medicine, Department of Microbiology, University of Medical Sciences and Technology (UMST), Sudan Accepted: June 28, 2021 Published online: June 30, 2021 Citaton: Hamid OM, Bayoumi MA (2021) A Retrospectve Study of Uropathogenic ESBL-producing Enterobacteriaceae among Hospitalized Patents, Khartoum-Sudan. Ann Microbiol Res 5(1):120-124 A Retrospective Study of Uropathogenic ESBL-producing Enterobacteriaceae among Hospitalized Patients, Khartoum-Sudan Omnia M Hamid 1 and Magdi A Bayoumi 2 * 1 Faculty of Medical Laboratory Sciences, University of Medical Sciences and Technology, Sudan 2 Faculty of Medicine, Department of Microbiology, University of Medical Sciences and Technology (UMST), Sudan Introduction Enterobacteriaceae member commonly related to hospital acquired UTI infectons, mainly catheter-related UTIs. They are caused partcularly by Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis isolates with high recurrence rates of increasing antmicrobial resistance among those uropathogens [1,2]. With the wide use of extended-spectrum cephalosporin's antbiotc in the healthcare setng, used as frst-line therapy for Enterobacteriaceae UTI, the major worry is the increased reports on the disseminaton of Hospital- Acquired UTI with ESBL-producing Enterobacteriaceae (ESBL-PE) that may limit available treatment optons, as they contribute signifcantly to the rapid disseminaton of resistant organisms and their genes [3,4]. The ESBLs are Research Article Abstract Uropathogenic extended-spectrum beta-lactamases-producing Enterobacteriaceae (ESBL-PE) remains a global cause of urinary tract infectons (UTIs) among hospitalized patents as these enzymes hydrolysing 3rd generaton cephalosporin’s (3rd GC). Those bacteria may not respond to therapy and thus further limitng antmicrobial selectons. This study aimed to detect uropathogenic Enterobacteriaceae producing ESBL genes and their antmicrobial resistance patern. Hundred and four (n=104) Enterobacteriaceae uropathogens were isolated in (2007-2008), from midstream urine (MSU) samples. In 2019, 54% of the recovered isolates were resistant to 3 rd GCs. The presence of ESBL-genes (blaTEM, blaSHV, and blaCTX –M genes) screened by multplex polymerase chain reacton (PCR). ESBL gene producing isolates were detected among 44.6%, and 36% of the ESBL-producing isolates were harbouring 2 ESBL genes. The common gene was the blaTEM, 40%, followed by 16% blaCTX-M and 8% blaSHV. The most dominant isolate was E. coli 60%, followed by K. pneumonia 36%, and E. aerogenes 4%. This study revealed that blaTEM was the most prevalent ESBL-PE uropathogens before 10 years in our region, although the current ESBL genes is CTX-M types exceeded SHV and TEM as the dominatng type, and is thought to involve clonal spread. Our community; needs molecular-based epidemiological studies to report the antmicrobial resistance genes in the Sudan region besides the origin of those genes. Keywords Extended-spectrum, ß-lactamases producing Enterobacteriaceae, uropathogens, bla-genes Abbreviatons MSU: Mid-stream urine; GNB: Gram-negatve bacteria; ESBL: Extended-spectrum β-lactamase; ESBL-PE: Extended- spectrum β-lactamase producing Enterobacteriaceae; 3 rd GC: 3 rd generaton cephalosporin’s; NCCLS: Natonal commitee on clinical laboratory standards; CLSI: Clinical laboratory standard insttute; UTI: Urinary tract infecton; MHA: Mueller Hinton agar; MDR: Multdrug resistance; Escherichia coli: E.coli; Klebsiella pneumoniae: K. pnuemoniae; Enterobacter aerogenes: E. aerogenes; Species: spp Check for updates