ORIENTAL JOURNAL OF CHEMISTRY www.orientjchem.org An International Open Access, Peer Reviewed Research Journal ISSN: 0970-020 X CODEN: OJCHEG 2023, Vol. 39, No.(3): Pg. 792-797 This is an Open Access article licensed under a Creative Commons license: Attribution 4.0 International (CC- BY). Published by Oriental Scientifc Publishing Company © 2018 Green Procedure for the synthesis of Copper Nanoparticles using Nerium oleander Leaf Extract: Characterizations and Applications HELEN MERINA ALBERT 1 * and C. ALOSIOUS GONSAGO 2 1 Department of Physics, Sathyabama Institute of Science and Technology, Chennai, India. 2 Department of Electronics Science, Mohamed Sathak College of Arts and Science, India. *Corresponding author Email: drhelenphy@gmail.com http://dx.doi.org/10.13005/ojc/390332 (Received: May 03, 2023; Accepted: June 06, 2023) ABSTRACT In the present study, Copper nanoparticles (Cu-NPs) are produced using Nerium oleander leaf extract via a green process. The prepared samples were examined by powder X-ray diffraction (P-XRD), ultraviolet-visible (UV-Vis) spectroscopy; Fourier transforms infrared (FTIR) spectroscopy, feld emission scanning electron microscopy (FESEM) and antibacterial screenings. According to XRD measurements, the Cu-NPs have a monoclinic form. According to the UV-Vis study, the minimal absorbance between 250 and 1000nm is due to by the formation of high-quality samples. The functional groups of Cu-NPs were verifed by FT-IR spectroscopy. According to the FE-SEM analysis, the Cu-NPs are clusters of smaller cylindrical objects with good uniformity. The antibacterial results show that the samples are fairly effective against Escherichia coli and Salmonella typhi bacterium. The fndings indicate that Cu-NPs can be used in the medical sector for surgical tools. Keywords: Green synthesis, XRD, FT-IR, FESEM, Antibacterial ability. INTRODUCTION Nanotechnology is the study and control of matter at sizes between 1 and 100 nanometers, where different phenomena alter novel applications. These materials' physical and chemical characteristics will be completely different from those of their bulk form at the nanoscale. Metal nanoparticles are currently very important due to their catalytic activity, optical and electrical, magnetic characteristics, and antibacterial capabilities. Metal-based nanobiotechnology has become increasingly popular due to its cutting-edge nature and a wide variety of applications in nearly all scientifc and technological felds, notably health and life sciences 1-3 . Due to their unique biological and mechanical characteristics, copper nanoparticles (Cu-NPs) have drawn the most attention among metal nanoparticles. Cu-NPs exhibit an electron correlation effect, spin dynamics, and high- temperature superconductivity. These attributes have greatly increased its applications as a heterogeneous catalyst, antimicrobial agent, drug delivery system, and antioxidant in the biomedical industry 4,5 .