FP08-05 NEUROGENESIS AND ANGIOGENESIS IN PANCREATIC CANCER EVOLVE CONCORDANTLY VIA DISTINCT TRANSCRIPTIONAL PROFILES I. E. Demir, I. Kröger, S. Teller, H. Friess and G. O. Ceyhan Department of Surgery, Technische Universitaet Muen- chen, Germany Introduction: Pancreatic cancer (PCa) is characterized by increased intratumoral nerve density and nerve hypertro- phy. Conversely, the density of vessels and tumor perfusion in PCa were reported to be decreased when compared to normal pancreas, creating a hypoxic tumor microenviron- ment. This observation raises the question whether neuro- genesis and angiogenesis in PCa behave oppositely or even suppress each other. Method: Human PCa specimens (UICC stages II-III, n = 57) were analyzed for the density of all (CD31 + ) ves- sels, all (S100 + ) nerves, and for the severity of neural in- vasion/NI and angioinvasion via cytokeratin-19 (CK19) triple immunolabeling. The transcriptomic profile of tumors with strong neurogenesis or strong angiogenesis was compared to those with little neuro- or angiogenesis via profiler PCR-arrays. Results: 41 out of 57 patients exhibited NI, whereas angioinvasion was detected in only one out of 57 patients. The mean vessel size and mean nerve size correlated positively (Sperman’s coefficient r = 0.43, p = 0.0013), and the total area of nerves increased in parallel with the total area of vessels in the analyzed specimens (r = 0.28, p = 0.04). The frequency of NI increased in concordance with the amount of tumor innervation (r = 0.25, p = 0.059). There was no association between vessel density and NI. Patients with more pronounced tumor angiogenesis had greater intratumoral amounts of matrix metalloproteinase-9 (MMP9), beta-III-integrin, and TgfbetaR1, whereas pa- tients with more neurogenesis exhibited intratumoral upregulation of interleukin-6, TrkA, Nr1I2, NGF, CX3CR1, and Neurotrophin-3. Conclusions: Angiogenesis and neurogenesis behave concordantly in PCa. Interleukin-6 and neurotrophic factors may be responsible for the remarkably high prevalence of NI in PCa. FP08-06 HIGH-THROUGH PUT MOLECULAR PROFILING OF MICROSATELLITE INSTABILITY IN PANCREATIC AND PERI-AMPULLARY CANCERS S. B. Koganti 1,2 , B. Nagari 1 and S. Regulagadda Adikesava 1 1 Gastrointestinal Surgery, Nizams Institute of Medical Sciences, India, and 2 BLHC, Albert Einstein College of Medicine, United States Background: Telomere abnormalities are among the earliest known events in the cascade of pancreatic and peri- ampullary cancer development. Marked telomere short- ening can be found in more than 70% with reported MSI (microsatellite instability) of 5e50% in these tumors. Very sparse literature exist that define tumor profiles and risk factors associated with MSI positivity. Aim: To screen for MSI in pancreatic&peri- ampullary cancers over a 5 year period and identify significant associated risk factors and define its prognostic significance. Material and methods: Tissue micro array was constructed for pancreatic &peri-ampullary cancers that included benign pancreatic tissue in correlation to different stages of adenocarcinoma. Immunohistochem- ical labelling was performed using antibodies to MSI markers. MSI positivity was defined a microsatellite variation at three or more of eight loci of BAT25, BAT26, D5S346, D2S123, and D17S250. Univariate analysis (fisher’s exact and chi-square test) was performed to determine the significance of MSI between tumorous and non-tumorous tissue. Different grades of the tumor as well as cancer phenotypes were studied to identify any correlation with MSI positivity. Odds ratio defined the strength of association and a p value of < 0.5 was considered significant. Conclusions: Overall 77 tumor samples (19 Pancreatic head, 58 Peri-ampullary cancers) were analysed using the Tissue microarray during the five year study period. MSI positivity strongly (76% vs 11%, Z = 3.324 , P < 0.03%) correlates with malignancy. Association of MSI positivity with age, Smoking &BMI was not significant. MSI+ tumors were more medullary type and poorly differenti- ated (32% vs 3% P < 0.008) and can have a predictive value comparable to traditional Clinicopathological features. FP08-07 PROGNOSTIC INDEX TO PREDICT SURVIVAL FOLLOWING PANCREATICODUODENECTOMY FOR MALIGNANCY B. V. Dasari 1 , K. Roberts 1 , J. Hodson 1 , L. Stevens 2 , A. Smith 2 , J. Isaac 1 , S. Hubscher 1 , R. Marudanayagam 1 , R. Sutcliffe 1 , P. Muiesan 1 and D. Mirza 1 1 Queen Elizabeth Hospital, and 2 St James’ University Hospital, United Kingdom Introduction: Site of tumour origin and lymph node me- tastases are important factors determining prognosis in pa- tients undergoing pancreaticoduodenectomy (PD) for adenocarcinomas arising from head of pancreas, ampulla, distal bile duct and duodenum. Additional prognostic in- formation is associated with the lymph node ratio (LNR). This study hypothesised that a prognostic index based on the significant clinic-pathological variables identified through statistical modelling could be used to create a predictive score for survival for each tumour type following PD. Methods: Patients who underwent PD between 2004 and 2013 were included. Univariable and multivariable (Cox regression) analyses were performed to identify predictors of survival, and a risk score was derived. The prognostic index (PI) was subsequently validated using an external patient cohort using AJCC staging system. Results: A total of 567 patients underwent either classical PD (10%) or pylorus preserving PD (90%). Tumour site (p < 0.001), tumour size (p = 0.002), T stage (p < 0.001), vascular involvement (p = 0.002), number of positive nodes HPB 2016, 18 (S1), e1ee384 e32 Electronic Poster Abstracts