Protective effects of Lactococcus lactis expressing alcohol dehydrogenase and acetaldehyde dehydrogenase on acute alcoholic liver injury in mice Yunbin Lyu 1 , Lei Zhong 1 , Yanan Liu 1 , Jing Lu 1 , Gisèle LaPointe 2 , Fengxia Lu 1 , Zhaoxin Lu 1* College of Food Science and Technology, Nanjing Agriculture University, Nanjing, P. R. China 1 , and Department of Food Science, University of Guelph, Guelph, Canada 2 Corresponding author: Zhaoxin Lu Email: fmb@njau.edu.cn Tel: +86-25-84396583 Fax: +86-25-84396583 Abstract Background: Alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) play important roles in alcohol metabolism. Therefore, a possible effective way to attenuate the alcoholic liver damage is the exogenous supply of these two enzymes in stomach as they might accelerate the oxidation of ethanol into nontoxic acetate. Results: ADH and ALDH were coexpressed in Lactococcus lactis NZ3900 and used as treatments towards acute alcoholic liver injury in mice. Intragastric ethanol Accepted Article This article is protected by copyright. All rights reserved. This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jctb.5521 Accepted Article