Research Article Volume 15 Issue 3 - February 2022 DOI: 10.19080/CRDOJ.2022.15.555913 Curr Res Diabetes Obes J Copyright © All rights are reserved by Vaishali R Undalea Curr Res Diabetes Obes J 15(3): CRDOJ.MS.ID.555913 (2022) 001 Investigation of Effect of Polyherbal Mixtures on Cafeteria Diet Induced Obesity in Wistar rats Komal V Umbarkar, Vaishali R Undalea*, Abhijeet S Kulkarni, Suchita Gupta, Sagar S Jadhav and Roshani R Pagar Department of Pharmacology, Dr. DY Patil Institute of Pharmaceutical Sciences & Research, India Submission: January 28, 2022; Published: February 09, 2022 *Corresponding author: Vaishali R Undalea, Department of Pharmacology, Dr. DY Patil Institute of Pharmaceutical Sciences & Research, India Abstract Background: To evaluate the anti-obesity activity of polyherbal mixture of selected herbs in cafeteria diet induced obesity in Wistar rats. Method: Hydro alcoholic extracts of herbal plant Annona muricatta, Momordica charantia, Vaccinium uliginosum were purchased and authenticated from Green Heaven India. The Acute Oral Toxicity study of polyherbal mixture was performed according to OECD guideline 423.The antiobesity effect of polyherbal mixture was evaluated in cafeteria diet (CD) induced obesity. The polyherbal mixture (PHM) at two different dose level (200 mg/kg and400 mg/kg BW) was administered orally for a period of 35 days and various parameters of anti-obesity were estimated. Result: The half maximal inhibitory concentration (IC50) values were estimated for combination of PHM in in vitro for antilipase activity. Oral administration of PHM for 35 days significantly decreases in calorie intake, bodyweight, cholesterol level and fasting glucose level. It showed that there is significant increase in parameters of liver function, BMI and Nasoanal length and level of white adipose tissue. Conclusion: The dose of 200 mg/kg and 400 mg/kg of PHM significantly reduced body weight and calorie intake indicating its anti- obesity effect. The mixture of PHM at both doses effectively improved serum lipids level and decreased serum blood glucose level suggesting its effect on carbohydrate and lipid metabolism. The decreased AST and ALT level signifies that the PHM do not interferes in liver function. The histopathological studies of liver tissue from PHM treated animals showed improvement in fatty liver and hepato-steatosis as compared to control group. From all above findings it is concluded that the PHM produces significant antiobesity effect in CFD induced obesity in Wistar rats at doses 200 & 400 mg/kg. Keywords: Obesity; Cafeteria diet; Acute oral toxicity; Hepato-steatosis; Antilipase activity Abbreviations: CD: Cafeteria Diet; PHM: Polyherbal Mixture; IC50: Half Maximal Inhibitory Concentration; NAFLD: Non- Alcoholic Fatty Liver Disease; IEAC: Institutional Animal Ethics Committee; CPCSEA: Committee for the Purpose of Control and Supervision of Experiments on Animals; CMC: Carboxy Methyl Cellulose; WAT: White Adipose Tissue; SEM; Standard Error of the Mean; BT: Bonferroni Test; P: Probability Introduction The major cause of morbidity and mortality is obesity worldwide in the 21 st Century [1]. Over the last 3 decades the morbidity rate of obesity has tripled worldwide. About 1.4 billion of people suffering from obesity and among them 200millions of men and 300millions of women were overweight [2]. In India, first four positions occupied in obesity states like Punjab, Kerala, Goa and Tamil Nadu (35%, 30%, 25% and 20%) [3]. The worldwide increase in obesity is because of changes in eating patterns and the consumption of high caloric diet. Stress, combined with overindulges and lethargy, can lead to obesity and this condition increases the frequency of most deadly diseases including diabetes mellitus (Type II), high blood pressure, coronary heart diseases, osteoarthritis, obstructive sleep disorders, non- alcoholic fatty liver disease (NAFLD) and certain cancer [1]. The occurrences of all these co morbidities are on rise in developed and developing countries stinging beyond the age and sex. Obesity builds up when there is irregular development of adipose tissue due to increase in fat cell size or fat cell number or combination thereof.. This is happened because of imbalance between energy input and energy output.