_____________________________________________________________________________________________________ *Corresponding author: E-mail: jonas.anayo@uniabuja.edu.ng; J. Adv. Biol. Biotechnol., vol. 25, no. 9, pp. 1-12, 2022 Journal of Advances in Biology & Biotechnology Volume 25, Issue 9, Page 1-12, 2022; Article no.JABB.90840 ISSN: 2394-1081 Evaluation of Healing and Microbial Responses to Surgical Wounds in Alloxan-induced Diabetic Rabbits Jonas Anayo Onah a* , Samuel Tanko Fadason b , Ebenezer Olaoluwa Abidoye b , Kwem Kadima c and Simon Ikechukwu Enem d a Department of Veterinary Surgery, University of Abuja, Nigeria. b Department of Veterinary Surgery and Radiology, Ahmadu Bello University, Zaria, Nigeria. c Veterinary Teaching Hospital, Ahmadu Bello University, Zaria, Nigeria. d Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Authors JAO, STF and KK designed the study. Authors EOA, JAO and SIE performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors JAO, STF, KK and EOA managed the analyses of the study. Authors JAO and STF managed the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JABB/2022/v25i9594 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/90840 Received 15/06/2022 Accepted 22/08/2022 Published 08/12/2022 ABSTRACT The wounds of diabetic patients are associated with delayed healing and microbial invasion. This study evaluated the healing and microbial responses to surgical wounds in diabetic rabbits. Sixteen (16) New Zealand White rabbits were used in this study. They were divided into 4 groups of 4 rabbits each, namely; A, B, C and D. A is the control (non-diabetic and non-wounded), B (diabetic and non-wounded), C (wounded and non-diabetic) and D (wounded and diabetic). Three (3) cm 2 skin wounds were created in a standard aseptic condition while diabetes was induced by Original Research Article