72 Bulletin UASVM, Veterinary Medicine 67(1)/2010 ISSN 1843-5270; Electronic ISSN 1843-5378 Diagnosis Techniques of the Neutropenia Syndrome – Immobility of the Leucocytes with Dogs Gabriel GÂJÂILĂ, Iuliana GÂJÂILĂ, Gabriel COTOR, Mimi DOBREA University of Agricultural Sciences and Veterinary Medicine Bucharest, Faculty of Veterinary Medicine, Splaiul Independentei 105, 5 th district imunogg@yahoo.com Abstract. Primary immunodeficits related to the phagocitary function, signaled in dogs, constitute a cause in the activation of certain variable, uncharacteristic clinical signs, with an uncertain therapeutic response. An immunodeficit condition framable in this group is the neutropenia syndrome – immobility of leucocytes which can be diagnosed through functional evaluation techniques of the neutrophiles. In this sense there was used the evaluation technique of migration through filtering layer and under agarose, the evaluation technique of the capacity of embedment of carbon particles and the evaluation technique of the total bactericide capacity. Keywords: phagocitosis, neutrophiles, migration, immunodeficit INTRODUCTION Primary immunodeficits signaled in dogs are affections determined mostly genetically, with unclear causality. In the group of functional deficits signaled in dogs’neutrofiles there is also the neutropenia syndrome – immobility of leucocytes, also called the „lazy” leucocyte syndrome. With no high incidence, the syndrome was signaled and diagnosed especially in people. With dogs, the anomaly can be categorized within the group of functional immunodeficits of the phagocitary cells, with a hereditary predisposition in the Irish setter and Doberman. Starting from the observations noted in the reference literature and the casuistry within the clinic of the Faculty of Veterinary Medicine – Bucharest, five Irish setters were tested so as to identify the functional tests which might lead to this diagnostic. MATERIAL AND METHODS The biologic material used was represented by five dogs of the Irish setter breed, 17-23 months old, unrelated, out of which three dogs displayed clinical signs of lymphadenitis, gingivitis and supurate dermatitis. The other two exemplars were clinically healthy in well-maintained condition. The samples collected for the diagnostic of the immunodeficit were venous blood collected on heparin in three phases at intervals of two weeks. The techniques used for diagnosing the immunodeficit mainly sought to establish the number of neutrofiles and their functional evaluation. With this objective, leucogramas were