Ө ƶҰƺƵǚ ¤ ǛNJ үDŽ ƵDŽ ƾƲҚƵǚǃ Ғƃ Ө ƾǂƵǚ ҒƶҰƹ 32 ơƀҰƵǚ ¤ ) (B əӨƞƵǚ . 2 ¤ 2014 1 Effect of some Physical Elicitors on some Secondary Metabolite Induction of Hypercom Triquetrifolium in Vitro Baan M.Abdulrazzak College of Science, Al-Mustansiriyah University/|Baghdad Email:Baan_twaij@yahoo.com Dr.Saadia H.Mahmood College of Science, Al-Mustansiriyah University/|Baghdad Dr.Kadhim M.Ibrahim College of Science, Al-Nahrian University/ Baghdad Received on: 8/11/2012 & Accepted on: 4/4/2013 ABSTRACT This project aimed to increase the production of some secondary metabolites using physical and chemical elicitors in tissue cultures of Hypercom triquetrifolium L.. The quality and quantity of photochemical were estimated using methanolic extracts of dried leaves and callus were analyses using HPLC. Callus was initiated on leaf discs cultured on Murashig and Skoog (MS) medium supplemented with 2,4-Dichlorophenoxy acetic acid (2,4-D) and Benzyl adenosine (BA) at concentrations of 0,0.1, 0.5, 2.0 or 5.0 mg/l for H. triquetrifolium callus. Results showed that the combination of 2,4-D at 0.1 mg/l with BA at 0.5 mg/l was the most effective for callus induction percentage reached 90%. The highest mean fresh weight reached 64.33 mg for H. triquetrfolium. The same combination was used for callus maintenance for plants. Results also showed an increase in the concentration of secondary metabolites in methanol extracts induced on leaves. Callus cultures induced on leaf discs were treated with some physical stimuli such as light, ultraviolet, the different exposure to photoperiod (dark for 24 hrs, 12 hrs light, 16 hrs light or 24 hrs light), the uv exposure time was 10 or 20 minutes. Result showed that there are significant differences between the various treatmeant, The best light exposure time caused an increase in the production of secondary metabolism was 12 hrs light for calli in both plants. Both exposure times (10 or 20 mint) induced the yield of secondary metabolites in callus cultures of H. triquetrifolium equally. ﺗﺄﺛﯿﺮ اﻟﻤﺤﻔﺰات ﺑﻌﺾ اﻟﻔﯿﺰﯾﺎﺋﯿﺔ ﻓﻲ إﻧﺘﺎج اﻟﺮوﺟﺔ ﻟﻨﺒﺎت اﻟﺜﺎﻧﻮي اﻻﯾﺾ ﻣﺮﻛﺒﺎت Hypericum triquetrifolium اﻟﺤﻲ اﻟﺠﺴﻢ ﺧﺎرج اﻟﺨﻼﺻﺔ اﻟﺾ اﻻﯾﺎت ﻣﺮﻛﺒﺾ ﺑﻌﺎج إﻧﺘﺎدة زﯾﺪف ﺑﮭﺎﻟﻲ اﻟﺤ اﻟﺒﺤﺚ اﺟﺮيﺎت ﻟﻨﺒﯿﺠﯿﺔ اﻟﻨﺴﺰارع اﻟﻤﻲ ﻓﺎﻧﻮي ﺜ ﺔ اﻟﺮوﺟHypericum triquetrifolium , ﺎت ﻣﺮﻛﺒﺪرت ﻗﻮﻋﻲ واﻟﻨﻲ اﻟﻜﻤﻞ ﺑﺎﻟﺘﺤﻠﯿﺎﻧﻮي اﻟﺜﺾ اﻻﯾ ﺟﮭﺎز ﺑﺎﺳﺘﻌﻤﺎلHPLC ﻞ اﻟﻜﺎﻣﺎت ﻟﻠﻨﺒﺔ اﻟﺠﺎﻓﻸوراق ﻟﺎﻧﻮﻟﻲ اﻟﻤﯿﺜﺘﺨﻠﺺ اﻟﻤﺴﻊ ﻣﺖ وﻗﻮرﻧ. ﻮلﺼ اﻟﺤﻢ ﺗ  اﻟﻜﻰ ﻋﻠﺎت اﻟﻨﺒوراق أﻦ ﻣءﺰاﺟ أﺬ ﺧﺄ ﺑﺲ ﺎﻟﻲﺋاﺬﻐ اﻟﻂ اﻟﻮﺳﻰ ﻋﻠﺎ وزراﻋﺘﮭ(MS) Murashige and Skoog ﺖﻤﻠ ﺷﻮ اﻟﻨﻤﺎتﻤﻈ ﻣﻨﻰ ﻋﻠﺎوي اﻟﺤ2,4-Dichlorophenoxy acetic acid ) (2,4-D وBenzyl adinenine ) BA ( ﺑﺎﻟﺘﺮ ﻛﯿﺰ0.1 و0.5 ﻐﻢ ﻣﻠ/ ﻮاﻟﻲ اﻟﺘﻰ ﻋﻠ ﻟﺘﺮ. ﺎت اﻟﻤﺮﻛﺒﺎج إﻧﺘﺎدة زﯾﺔﯿﻐ وﺑ https://doi.org/10.30684/etj.32.2B.20 2412-0758/University of Technology-Iraq, Baghdad, Iraq This is an open access article under the CC BY 4.0 license http://creativecommons.org/licenses/by/4.0