ISSN: 2410-9649 Mouhamad et al / Chemistry International 9(1) (2013) 15-20 iscientic.org. 15 http://bosaljournals.com/chemint editorci@bosaljournals.com Article type: Research article Article history: Received June 2022 Accepted November 2022 January 2023 Issue Keywords: Arabidopsis thaliana Sesbania grandiflora CYP2E1 Cesium Uranium Strontium Genetically modified S. grandiflora and A. thaliana confer P450 2EI enzyme were examined for accumulating Cs, Sr and U. The absorption of these radionuclides ( 137 Cs, 90 Sr and 238 U) by the generated transgenic plants was analyzed through quantitative gamma assay. Both transgenic plants which derived from homozygous T3 were grown in soil containing 137 Cs 17600 Bq/g, 90 Sr 1477 mg/kg and supplemented with uranium at 25 mM. Transgenic plants with a high CYP2E1 expression level as proved by Western Blot analysis that the absorption of radioactive nuclides increased significantly in comparison with wild type plants or GUS transgenic plants (control). Arabidopsis and Sesbania transgenic plants were able to accumulate 80% and 70%, respectively of the available 238 U versus control plants and thus, 238 U concentration was significantly decreased in the soil by 50% and 60%, respectively versus the soils cultivated with control or wild type plants. Similarly, 90 Sr accumulation levels recorded 70% and 60% increase in transgenic Arabidopsis and Sesbania plants, respectively which led to an important decrease in 90 Sr by 50%. Similarly, 70% and 60% increases in 137 Cs levels were recorded in transgenic plants, respectively leading to 40% and 30% decreases in 137 Cs in correlated soils. The CYP2E1 gene improves plants ability to resist radioactive pollutants and may open a new avenue for resisting other pollutants such as U, Cs and Sr through the utilization and deployment of transgenic flowering plants. However, the mechanism by which CYP2E1 enhances the uptake of these radioactive nuclides still to be investigated and examined. © 2023 International Scientific Organization: All rights reserved. Capsule Summary: Genetically modified S. grandiflora and A. thaliana were examined for accumulating Cs, Sr and U, which accumulate the radiative elements and the CYP2E1 gene improve the plants ability to resist and grow in polluted soil. Cite This Article As: R. Mouhamad, K. Ibrahim and A. Al-Daoude. Enhanced translocation of uranium, strontium and cesium by transgenic Sesbania and Arabidopsis plants harboring the rabbit CYP2E1 gene. Chemistry International 9(1) (2013) 15-20. https://doi.org/10.5281/zenodo.7317656 INTRODUCTION Toxic metals interfere with biochemical and homeostatic processes in the cell through the production of free radicals (Dushenkov, 2003). The last few decades of wars in Kosovo, Iraq, and some other places have resulted in a depleted uranium (DU) contamination in addition to and other toxic Chemistry International 9(1) (2013) 15-20 Enhanced translocation of uranium, strontium and cesium by transgenic Sesbania and Arabidopsis plants harboring the rabbit CYP2E1 gene Raghad Mouhamad 1, *, Kadhim Ibrahim 2 and Antonious Al-Daoude 3 1 Ministry of Science and Technology, Baghdad, Iraq 2 Al-Nahrain University, Baghdad, Iraq 3 Atomic Energy Commission, Damascus, P.O. Box 6091, Syria *Corresponding author’s E. mail: raghad1974@yahoo.com ARTICLE INFO ABSTRACT